{"doi":"10.1074/jbc.ra119.011367","title":"A novel mechanism of ribonuclease regulation: GcvB and Hfq stabilize the mRNA that encodes RNase BN/Z during exponential phase","abstract":null,"journal":"Journal of Biological Chemistry","year":2019,"id":639888,"datarank":0.40620753016533157,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.0,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1662863,"name":"Angelica Previero","orcid":null,"position":1,"is_corresponding":false},{"id":777745,"name":"Murray P. Deutscher","orcid":null,"position":2,"is_corresponding":false},{"id":957752,"name":"Hua Chen","orcid":"0000-0003-0676-3079","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A novel mechanism of ribonuclease regulation: GcvB and Hfq stabilize the mRNA that encodes RNase BN/Z during exponential phase","abstract":"RNase BN, the Escherichia coli RNase Z family member, plays a limited role in tRNA metabolism, in contrast to most other organisms. However, RNase BN does act on 6S RNA, the global transcription regulator, degrading it in exponential-phase cells and maintaining it at low levels during this phase of growth. RNase BN levels decrease in stationary-phase cells, leading to elevation of 6S RNA and subsequent regulation of RNA polymerase. These findings were the first indication that RNase BN itself is growth phase–regulated. Here, we analyze the mechanism of this regulation of RNase BN. We find that RNase BN decreases in stationary phase because its mRNA becomes unstable, due primarily to its degradation by RNase E. However, in exponential-phase cells rbn mRNA is stabilized due to binding by the sRNA, GcvB, and the protein, Hfq, which reduce cleavage by RNase E. Because the amount of GcvB decreases in stationary phase, rbn mRNA is less protected and becomes increasingly unstable resulting in reduction in the amount of RNase BN. The small RNA-dependent, positive regulation of RNase BN in exponential-phase cells is the first example of this novel mechanism for RNase regulation.","is_dataset_classified":null,"base_score":2.70805020110221,"endowment":2.70805020110221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31744883","pmcid":"PMC6937589","openalex_id":"https://openalex.org/W2989678952","authors":[],"funders":[{"funder_name":"The National Institutes of Health","grant_id":"GM016317","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"F32 GM016317","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM016317","title":null}],"total_grants":3,"fwci":1.6107,"citation_percentile":0.84451164,"influential_citations":0,"citation_trend":[{"year":2020,"count":2},{"year":2021,"count":6},{"year":2022,"count":2},{"year":2023,"count":2},{"year":2024,"count":2}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.jbc.org/article/S0021925820300211/pdf","host_type":"journal"},{"url":"http://www.jbc.org/article/S0021925820300211/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820300211?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820300211?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.RA119.011367","host_type":"publisher"},{"url":"https://doi.org/10.1074/jbc.ra119.011367","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/31744883","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6937589","host_type":"repository"}],"fields_of_study":["Bacterial Genetics and Biotechnology","RNA and protein synthesis mechanisms","Bacteriophages and microbial interactions"],"mesh_terms":["Base Sequence","Endoribonucleases","Escherichia coli","Exoribonucleases","Protein Binding","RNA, Messenger","RNA Stability","Transcription Initiation Site","Escherichia coli Proteins","Host Factor 1 Protein","RNA, Small Untranslated"],"keywords":["RNase P","RNase MRP","RNase H","Ribonuclease","RNA","Messenger RNA","RNase PH","Biology","Ribonuclease III","Molecular biology","Transcription (linguistics)","Cell biology","Chemistry","Gene","Biochemistry","RNA interference","Bacteria","Post-transcriptional regulation","mRNA stability","Mrna Decay","Regulatory Rna","Growth-phase Regulation"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T03:41:24.068987Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}