{"doi":"10.1074/jbc.ra119.011185","title":"The noncanonical small heat shock protein HSP-17 from Caenorhabditis elegans is a selective protein aggregase","abstract":"Small heat shock proteins (sHsps) are conserved, ubiquitous members of the proteostasis network. Canonically, they act as \"holdases\" and buffer unfolded or misfolded proteins against aggregation in an ATP-independent manner. Whereas bacteria and yeast each have only two sHsps in their genomes, this number is higher in metazoan genomes, suggesting a spatiotemporal and functional specialization in higher eukaryotes. Here, using recombinantly expressed and purified proteins, static lightscattering analysis, and disaggregation assays, we report that the noncanonical sHsp HSP-17 of Caenorhabditis elegans facilitates aggregation of model substrates, such as malate dehydrogenase (MDH), and inhibits disaggregation of luciferase in vitro. Experiments with fluorescently tagged HSP-17 under the control of its endogenous promoter revealed that HSP-17 is expressed in the digestive and excretory organs, where its overexpression promotes the aggregation of polyQ proteins and of the endogenous kinase KIN-19. Systemic depletion of hsp-17 shortens C. elegans lifespan and severely reduces fecundity and survival upon prolonged heat stress. HSP-17 is an abundant protein exhibiting opposing chaperone activities on different substrates, indicating that it is a selective protein aggregase with physiological roles in development, digestion, and osmoregulation.","journal":"Journal of Biological Chemistry","year":2020,"id":85978,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9615,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":212808,"name":"Dmytro Puchkov","orcid":"0000-0001-8341-4847","position":1,"is_corresponding":false},{"id":440603,"name":"Irving Rosas-Brugada","orcid":null,"position":2,"is_corresponding":false},{"id":440604,"name":"Linda Bergemann","orcid":null,"position":3,"is_corresponding":false},{"id":440605,"name":"Ulrike Rieprecht","orcid":null,"position":4,"is_corresponding":false},{"id":91609,"name":"Janine Kirstein","orcid":"0000-0003-4990-2497","position":5,"is_corresponding":false},{"id":323382,"name":"Manuel Iburg","orcid":"0000-0002-1073-9023","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":null,"created_at":"2026-07-18T21:58:41.997408Z","pmid":"32001616","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}