{"doi":"10.1074/jbc.ra119.011034","title":"The plastidial Arabidopsis thaliana NFU1 protein binds and delivers [4Fe-4S] clusters to specific client proteins","abstract":"Proteins incorporating iron–sulfur (Fe-S) co-factors are required for a plethora of metabolic processes. Their maturation depends on three Fe-S cluster assembly machineries in plants, located in the cytosol, mitochondria, and chloroplasts. After de novo formation on scaffold proteins, transfer proteins load Fe-S clusters onto client proteins. Among the plastidial representatives of these transfer proteins, NFU2 and NFU3 are required for the maturation of the [4Fe-4S] clusters present in photosystem I subunits, acting upstream of the high-chlorophyll fluorescence 101 (HCF101) protein. NFU2 is also required for the maturation of the [2Fe-2S]-containing dihydroxyacid dehydratase, important for branched-chain amino acid synthesis. Here, we report that recombinant Arabidopsis thaliana NFU1 assembles one [4Fe-4S] cluster per homodimer. Performing co-immunoprecipitation experiments and assessing physical interactions of NFU1 with many [4Fe-4S]-containing plastidial proteins in binary yeast two-hybrid assays, we also gained insights into the specificity of NFU1 for the maturation of chloroplastic Fe-S proteins. Using bimolecular fluorescence complementation and in vitro Fe-S cluster transfer experiments, we confirmed interactions with two proteins involved in isoprenoid and thiamine biosynthesis, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase and 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase, respectively. An additional interaction detected with the scaffold protein SUFD enabled us to build a model in which NFU1 receives its Fe-S cluster from the SUFBC 2 D scaffold complex and serves in the maturation of specific [4Fe-4S] client proteins. The identification of the NFU1 partner proteins reported here more clearly defines the role of NFU1 in Fe-S client protein maturation in Arabidopsis chloroplasts among other SUF components.","journal":"Journal of Biological Chemistry","year":2020,"id":75187,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9529,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":392688,"name":"Jonathan Przybyla‐Toscano","orcid":"0000-0002-8053-6037","position":1,"is_corresponding":false},{"id":392689,"name":"Florence Vignols","orcid":"0000-0002-2031-0407","position":2,"is_corresponding":false},{"id":392690,"name":"Nathalie Berger","orcid":"0000-0003-0477-4903","position":3,"is_corresponding":false},{"id":392691,"name":"Tamanna Azam","orcid":"0000-0002-6370-1870","position":4,"is_corresponding":false},{"id":392692,"name":"Loïck Christ","orcid":"0000-0003-1452-8001","position":5,"is_corresponding":false},{"id":394029,"name":"Véronique Santoni","orcid":null,"position":6,"is_corresponding":false},{"id":392693,"name":"Hui-Chen Wu","orcid":"0000-0002-4991-7616","position":7,"is_corresponding":false},{"id":394030,"name":"Tiphaine Dhalleine","orcid":null,"position":8,"is_corresponding":false},{"id":392694,"name":"Michael K. Johnson","orcid":"0000-0002-8479-5810","position":9,"is_corresponding":false},{"id":392695,"name":"Christian Dubos","orcid":"0000-0001-5486-3643","position":10,"is_corresponding":false},{"id":392696,"name":"Jérémy Couturier","orcid":"0000-0003-3127-2669","position":11,"is_corresponding":false},{"id":392697,"name":"Nicolas Rouhier","orcid":"0000-0002-2036-7884","position":12,"is_corresponding":false},{"id":394028,"name":"Mélanie Roland","orcid":null,"position":0,"is_corresponding":true}],"reference_count":72,"raw_metadata":null,"created_at":"2026-07-18T21:46:33.313355Z","pmid":"31911438","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}