{"doi":"10.1074/jbc.ra119.010984","title":"A substitution in cGMP-dependent protein kinase 1 associated with aortic disease induces an active conformation in the absence of cGMP","abstract":", we did not detect increased autophosphorylation of the PKG1α or PKG1β RQ variant isolated from transiently transfected 293T cells, indicating that increased basal activity of the RQ variants in cells was not driven by PKG1 autophosphorylation. Replacement of Arg-177 in PKG1α with alanine or methionine also increased basal activity. PKG1 exists as a parallel homodimer linked by an N-terminal leucine zipper, and we show that the WT chain in WT-RQ heterodimers partly reduces basal activity of the RQ chain. Using hydrogen/deuterium-exchange MS, we found that the RQ substitution causes PKG1β to adopt an active conformation in the absence of cGMP, similar to that of cGMP-bound WT enzyme. We conclude that the RQ substitution in PKG1 increases its basal activity by disrupting the formation of an inactive conformation.","journal":"Journal of Biological Chemistry","year":2020,"id":72488,"datarank":0.6503216803951458,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.23443337205917855,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.23443337205917855,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":12,"citers_with_citation_signal":9,"citers_with_endowment":9,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9564,"is_data_producer":true,"deposit_databanks":{"PDB":["2QCS"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":384009,"name":"Sahar Aminzai","orcid":null,"position":1,"is_corresponding":false},{"id":384010,"name":"Tingfei Hu","orcid":null,"position":2,"is_corresponding":false},{"id":384011,"name":"Amatya Taran","orcid":null,"position":3,"is_corresponding":false},{"id":283942,"name":"Sheng Li","orcid":"0000-0002-9073-6809","position":4,"is_corresponding":false},{"id":382486,"name":"Choel Kim","orcid":"0000-0002-3152-0020","position":5,"is_corresponding":false},{"id":374130,"name":"Renate B. Pilz","orcid":"0000-0003-4555-3541","position":6,"is_corresponding":false},{"id":374128,"name":"Darren E. Casteel","orcid":"0000-0002-7673-6597","position":7,"is_corresponding":false},{"id":384008,"name":"Matthew H. Chan","orcid":null,"position":0,"is_corresponding":true}],"reference_count":46,"raw_metadata":null,"created_at":"2026-07-18T21:45:09.279825Z","pmid":"32506052","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}