{"doi":"10.1074/jbc.ra117.001143","title":"Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments","abstract":null,"journal":"Journal of Biological Chemistry","year":2018,"id":605250,"datarank":0.5101796072493234,"base_score":3.4011973816621555,"endowment":3.4011973816621555,"self_citation_contribution":0.5101796072493234,"citation_network_contribution":0.0,"self_endowment_contribution":0.5101796072493234,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":29,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":478414,"name":"Kyle Kaniecki","orcid":null,"position":1,"is_corresponding":false},{"id":1553236,"name":"YoungHo Kwon","orcid":null,"position":2,"is_corresponding":false},{"id":278336,"name":"Patrick Sung","orcid":"0000-0003-1396-9040","position":3,"is_corresponding":false},{"id":247324,"name":"Eric C. Greene","orcid":"0000-0002-7387-824X","position":4,"is_corresponding":false},{"id":278334,"name":"J. Brooks Crickard","orcid":"0000-0002-5162-7466","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments","abstract":"During meiosis, the two DNA recombinases Rad51 and Dmc1 form specialized presynaptic filaments that are adapted for performing recombination between homologous chromosomes. There is currently a limited understanding of how these two recombinases are organized within the meiotic presynaptic filament. Here, we used single molecule imaging to examine the properties of presynaptic complexes composed of both Rad51 and Dmc1. We demonstrate that Rad51 and Dmc1 have an intrinsic ability to self-segregate, even in the absence of any other recombination accessory proteins. Moreover, we found that the presence of Dmc1 stabilizes the adjacent Rad51 filaments, suggesting that cross-talk between these two recombinases may affect their biochemical properties. Based upon these findings, we describe a model for the organization of Rad51 and Dmc1 within the meiotic presynaptic complex, which is also consistent with in vivo observations, genetic findings, and biochemical expectations. This model argues against the existence of extensively intermixed filaments, and we propose that Rad51 and Dmc1 have intrinsic capacities to form spatially distinct filaments, suggesting that additional recombination cofactors are not required to segregate the Rad51 and Dmc1 filaments.","is_dataset_classified":null,"base_score":3.4011973816621555,"endowment":3.4011973816621555,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29382724","pmcid":null,"openalex_id":"https://openalex.org/W2793661078","authors":[],"funders":[{"funder_name":"Office of Extramural Research, National Institutes of Health (OER)","grant_id":"R35GM118026","title":null},{"funder_name":"Office of Extramural Research, National Institutes of Health (OER)","grant_id":"R01ES007061","title":null},{"funder_name":"Office of Extramural Research, National Institutes of Health (OER)","grant_id":"RO1ES015632","title":null},{"funder_name":"Damon Runyon Cancer Research Foundation (Cancer Research Fund of the Damon Runyon-Walter Winchell Foundation)","grant_id":"DRG 2310–17","title":null},{"funder_name":"NIEHS NIH HHS","grant_id":"R01 ES015632","title":null}],"total_grants":5,"fwci":1.4838,"citation_percentile":0.82113942,"influential_citations":0,"citation_trend":[{"year":2018,"count":8},{"year":2019,"count":3},{"year":2020,"count":5},{"year":2021,"count":2},{"year":2022,"count":4},{"year":2023,"count":2},{"year":2024,"count":2},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.jbc.org/article/S0021925820410397/pdf","host_type":"journal"},{"url":"http://www.jbc.org/article/S0021925820410397/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820410397?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820410397?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.RA117.001143","host_type":"publisher"},{"url":"https://doi.org/10.1074/jbc.ra117.001143","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29382724","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5858004","host_type":"repository"}],"fields_of_study":["DNA Repair Mechanisms","DNA and Nucleic Acid Chemistry","CRISPR and Genetic Engineering","Cell Cycle Proteins","DNA, Single-Stranded","DNA-Binding Proteins","Meiosis","Microscopy, Fluorescence","Rad51 Recombinase","Recombination, Genetic","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins"],"mesh_terms":["DNA-Binding Proteins","DNA, Single-Stranded","Meiosis","Microscopy, Fluorescence","Recombination, Genetic","Saccharomyces cerevisiae","Cell Cycle Proteins","Saccharomyces cerevisiae Proteins","Rad51 Recombinase"],"keywords":["Recombinase","RAD51","Homologous recombination","Biology","Cell biology","Meiosis","Genetic recombination","Protein filament","Genetics","DNA","Biophysics","Recombination","Gene","DNA binding protein","DNA curtains","DNA recombination","DNA repair","Dmc1","presynaptic complex","presynaptic filament","single molecule","single-molecule biophysics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T02:00:26.579703Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}