{"doi":"10.1074/jbc.m603800200","title":"CDCA4 Is an E2F Transcription Factor Family-induced Nuclear Factor That Regulates E2F-dependent Transcriptional Activation and Cell Proliferation","abstract":null,"journal":"Journal of Biological Chemistry","year":2006,"id":684106,"datarank":0.6892679775201885,"base_score":4.59511985013459,"endowment":4.59511985013459,"self_citation_contribution":0.6892679775201885,"citation_network_contribution":0.0,"self_endowment_contribution":0.6892679775201885,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":98,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":6,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1787196,"name":"Yuya Goto","orcid":null,"position":1,"is_corresponding":false},{"id":1787197,"name":"Ryuji Ikeda","orcid":null,"position":2,"is_corresponding":false},{"id":457240,"name":"Kazunari K. Yokoyama","orcid":"0000-0001-8508-7587","position":3,"is_corresponding":false},{"id":208524,"name":"Kenichi Yoshida","orcid":null,"position":4,"is_corresponding":false},{"id":1787195,"name":"Reiko Hayashi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"CDCA4 Is an E2F Transcription Factor Family-induced Nuclear Factor That Regulates E2F-dependent Transcriptional Activation and Cell Proliferation","abstract":"The TRIP-Br1/p34(SEI-1) family proteins participate in cell cycle progression by coactivating E2F1- or p53-dependent transcriptional activation. Here, we report the identification of human CDCA4 (also know as SEI-3/Hepp) as a novel target gene of transcription factor E2F and as a repressor of E2F-dependent transcriptional activation. Analysis of CDCA4 promoter constructs showed that an E2F-responsive sequence in the vicinity of the transcription initiation site is necessary for the E2F1-4-induced activation of CDCA4 gene transcription. Chromatin immunoprecipitation analysis demonstrated that E2F1 and E2F4 bound to an E2F-responsive sequence of the human CDCA4 gene. Like TRIP-Br1/p34(SEI-1) and TRIP-Br2 (SEI-2), the transactivation domain of CDCA4 was mapped within C-terminal acidic region 175-241. The transactivation function of the CDCA4 protein was inhibited by E2F1-4 and DP2, but not by E2F5-8. Inhibition of CDCA4 transactivation activity by E2F1 partially interfered with retinoblastoma protein overexpression. Conversely, CDCA4 suppressed E2F1-3-induced reporter activity. CDCA4 (but not acidic region-deleted CDCA4) suppressed E2F1-regulated gene promoter activity. These findings suggest that the CDCA4 protein functions as a suppressor at the E2F-responsive promoter. Small interfering RNA-mediated knockdown of CDCA4 expression in cancer cells resulted in up-regulation of cell growth rates and DNA synthesis. The CDCA4 protein was detected in several human cells and was induced as cells entered the G1/S phase of the cell cycle. Taken together, our results suggest that CDCA4 participates in the regulation of cell proliferation, mainly through the E2F/retinoblastoma protein pathway.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":6,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16984923","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.jbc.org/article/S002192582068111X/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S002192582068111X?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S002192582068111X?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.M603800200","host_type":"publisher"},{"url":"http://www.jbc.org/content/281/47/35633.full.pdf","host_type":"Unpaywall"}],"fields_of_study":[],"mesh_terms":["Cell Line, Tumor","Hela Cells","Cell Nucleus","Humans","Cell Cycle Proteins","Retinoblastoma Protein","Cell Cycle","Cell Proliferation","Gene Expression Regulation","Amino Acid Sequence","Sequence Homology, Amino Acid","Tissue Distribution","Molecular Sequence Data","E2F Transcription Factors","Transcriptional Activation"],"keywords":[],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.16607042.v1","title":"Additional file 2 of Prenatal risk factors and neonatal DNA methylation in very preterm infants","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.16607042","title":"Additional file 2 of Prenatal risk factors and neonatal DNA methylation in very preterm infants","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.16607045.v1","title":"Additional file 3 of Prenatal risk factors and neonatal DNA methylation in very preterm infants","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.16607045","title":"Additional file 3 of Prenatal risk factors and neonatal DNA methylation in very preterm infants","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.17004282.v1","title":"Additional file 1 of MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression","publisher":"figshare","resource_type":"Image"},{"doi":"10.6084/m9.figshare.17004282","title":"Additional file 1 of MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression","publisher":"figshare","resource_type":"Image"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T13:19:04.992763Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}