{"doi":"10.1074/jbc.m400892200","title":"The Basic Leucine Zipper Domain of c-Jun Functions in Transcriptional Activation through Interaction with the N Terminus of Human TATA-binding Protein-associated Factor-1 (Human TAFII250)","abstract":null,"journal":"Journal of Biological Chemistry","year":2004,"id":686359,"datarank":0.4566783656585135,"base_score":3.044522437723423,"endowment":3.044522437723423,"self_citation_contribution":0.4566783656585135,"citation_network_contribution":0.0,"self_endowment_contribution":0.4566783656585135,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1793122,"name":"Tuan N. Nguyen","orcid":null,"position":1,"is_corresponding":false},{"id":166746,"name":"Shelly K. Galasinski","orcid":null,"position":2,"is_corresponding":false},{"id":166727,"name":"James A. Goodrich","orcid":null,"position":3,"is_corresponding":false},{"id":166744,"name":"Tricia N. Lively","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The Basic Leucine Zipper Domain of c-Jun Functions in Transcriptional Activation through Interaction with the N Terminus of Human TATA-binding Protein-associated Factor-1 (Human TAFII250)","abstract":"We previously reported that c-Jun binds directly to the N-terminal 163 amino acids of Homo sapiens TATA-binding protein-associated factor-1 (hsTAF1), causing a derepression of transcription factor IID (TFIID)-driven transcription (Lively, T. N., Ferguson, H. A., Galasinski, S. K., Seto, A. G., and Goodrich, J. A. (2001) J. Biol. Chem. 276, 25582-25588). This region of hsTAF1 binds TATA-binding protein to repress TFIID DNA binding and transcription. Here we show that the basic leucine zipper domain of c-Jun, which allows for DNA binding and homodimerization, is necessary and sufficient for interaction with hsTAF1. Interestingly, the isolated basic leucine zipper domain of c-Jun was able to derepress TFIID-directed basal transcription in vitro. Moreover, when the N-terminal region of hsTAF1 was added to in vitro transcription reactions and overexpressed in cells, it blocked c-Jun activation. c-Fos, another basic leucine zipper protein, did not interact with hsTAF1, but c-Fos/c-Jun heterodimers did bind the N terminus of hsTAF1. Our studies show that, in addition to dimerization and DNA binding, the well characterized basic leucine zipper domain of c-Jun functions in transcriptional activation by binding to the N terminus of hsTAF1 to derepress transcription.","is_dataset_classified":null,"base_score":3.044522437723423,"endowment":3.044522437723423,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15087451","pmcid":null,"openalex_id":"https://openalex.org/W1992545443","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM008759","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM07135","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM08759","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM142607","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM-55235","title":null}],"total_grants":5,"fwci":0.5069,"citation_percentile":0.61697985,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1},{"year":2016,"count":2},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2024,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://www.sciencedirect.com/science/article/pii/S0021925820856075/pdf","host_type":"journal"},{"url":"https://www.sciencedirect.com/science/article/pii/S0021925820856075/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820856075?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820856075?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.M400892200","host_type":"publisher"},{"url":"https://doi.org/10.1074/jbc.m400892200","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15087451","host_type":"repository"}],"fields_of_study":["interferon and immune responses","Genomics and Chromatin Dynamics","RNA Research and Splicing","Amino Acid Sequence","Animals","COS Cells","DNA","Dimerization","Dose-Response Relationship, Drug","Glutathione Transferase","Histone Acetyltransferases","Humans","Leucine Zippers","Models, Biological","Molecular Sequence Data","Peptides","Plasmids","Promoter Regions, Genetic","Protein Binding","Protein Structure, Tertiary","Proto-Oncogene Proteins c-fos","Proto-Oncogene Proteins c-jun","Recombinant Fusion Proteins","Salts","TATA-Binding Protein Associated Factors","Transcription Factor TFIID","Transcription, Genetic","Transcriptional Activation"],"mesh_terms":["Amino Acid Sequence","Animals","DNA","Dose-Response Relationship, Drug","Glutathione Transferase","Humans","Models, Biological","Molecular Sequence Data","Peptides","Plasmids","Promoter Regions, Genetic","Protein Binding","Recombinant Fusion Proteins","Salts","Transcription, Genetic","Transcriptional Activation","Leucine Zippers","Proto-Oncogene Proteins c-jun","Proto-Oncogene Proteins c-fos","Protein Structure, Tertiary","Dimerization","COS Cells","Transcription Factor TFIID","TATA-Binding Protein Associated Factors","Histone Acetyltransferases"],"keywords":["Leucine zipper","Basic helix-loop-helix leucine zipper transcription factors","bZIP domain","TAF1","ATF3","TAF2","TATA-binding protein","Transcription factor","TATA box","Transcription factor II A","General transcription factor","Activating transcription factor 2","Biology","TATA-Box Binding Protein","Molecular biology","Transcription factor II D","Activating transcription factor","CREB1","Transcription (linguistics)","Coiled coil","Cell biology","DNA-binding protein","Biochemistry","CREB","Enhancer","Gene","Promoter","RNA","RNA polymerase","Gene expression"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T19:17:20.686664Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}