{"doi":"10.1074/jbc.m400032200","title":"Human Kaposi's Sarcoma Herpesvirus Processivity Factor-8 Functions as a Dimer in DNA Synthesis","abstract":null,"journal":"Journal of Biological Chemistry","year":2004,"id":627708,"datarank":0.5495342469194471,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"self_citation_contribution":0.5495342469194471,"citation_network_contribution":0.0,"self_endowment_contribution":0.5495342469194471,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":38,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":831083,"name":"Kai Lin","orcid":"0000-0003-2186-7466","position":1,"is_corresponding":false},{"id":445796,"name":"Robert P. Ricciardi","orcid":"0000-0002-2206-3657","position":2,"is_corresponding":false},{"id":1500274,"name":"Xulin Chen","orcid":"0009-0000-6682-0472","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Human Kaposi's Sarcoma Herpesvirus Processivity Factor-8 Functions as a Dimer in DNA Synthesis","abstract":"In the absence of other proteins, the DNA polymerase (Pol-8) of Kaposi's sarcoma herpesvirus incorporates only several nucleotides from a primer template. However, association with the Kaposi's sarcoma herpesvirus processivity factor (PF-8) enables Pol-8 to incorporate thousands of nucleotides. Unlike the well described sliding clamp processivity factors, eukaryotic proliferating cell nuclear antigen and Escherichia coli beta-subunit, PF-8 and other herpesvirus processivity factors do not require a clamp loader or ATP to bind to template DNA. To begin to understand the mechanism used by PF-8 to achieve processivity, we have now purified PF-8 and demonstrated that it is a dimer both in solution and on the DNA. Mutational analysis of the PF-8 protein (396R) indicates that residues between 277 and 304 as well as the N-terminal 21 amino acids are required for dimerization. The results further correlate PF-8 dimerization with binding to Pol-8 and stabilizing Pol-8 on primer template. Notably, although removal of only 26 residues from the C terminus of PF-8 does not affect its ability to form dimers on DNA or to bind Pol-8, only short DNA chains (<100 nucleotides) are synthesized. This indicates that full-length PF-8 is necessary to enable Pol-8 to incorporate thousands of nucleotides. Interestingly, cross-linking of the processivity factor UL44 of cytomegalovirus reveals that it is a dimer in solution also.","is_dataset_classified":null,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15075322","pmcid":null,"openalex_id":"https://openalex.org/W2049816025","authors":[],"funders":[],"total_grants":0,"fwci":1.3168,"citation_percentile":0.7945277,"influential_citations":0,"citation_trend":[{"year":2013,"count":4},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2020,"count":2},{"year":2021,"count":3},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.jbc.org/article/S0021925820732795/pdf","host_type":"journal"},{"url":"http://www.jbc.org/article/S0021925820732795/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820732795?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820732795?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.M400032200","host_type":"publisher"},{"url":"https://doi.org/10.1074/jbc.m400032200","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15075322","host_type":"repository"}],"fields_of_study":["Viral-associated cancers and disorders","Cytomegalovirus and herpesvirus research","Herpesvirus Infections and Treatments"],"mesh_terms":["Adenosine Triphosphate","Cell Nucleus","Chromatography, Gel","Cross-Linking Reagents","Cytomegalovirus","DNA","DNA Mutational Analysis","Dose-Response Relationship, Drug","Electrophoresis, Polyacrylamide Gel","Escherichia coli","Herpesviridae","Models, Biological","Mutation","Precipitin Tests","Protein Binding","Recombinant Proteins","Sarcoma, Kaposi","Transcription, Genetic","Protein Biosynthesis","Viral Proteins","Blotting, Western","Gene Deletion","Protein Structure, Tertiary","Dimerization","Biotinylation"],"keywords":["Processivity","DNA polymerase","Primer (cosmetics)","DNA clamp","DNA","DNA polymerase delta","Nucleotide","Biology","Polymerase","Primase","Molecular biology","DNA polymerase II","Chemistry","Biochemistry","Reverse transcriptase","RNA","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T18:34:49.061627Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}