{"doi":"10.1074/jbc.m309682200","title":"Manduca sexta Serpin-3 Regulates Prophenoloxidase Activation in Response to Infection by Inhibiting Prophenoloxidase-activating Proteinases","abstract":null,"journal":"Journal of Biological Chemistry","year":2003,"id":664377,"datarank":7.461900160235528,"base_score":5.204006687076795,"endowment":5.204006687076795,"self_citation_contribution":0.7806010030615194,"citation_network_contribution":6.681299157174008,"self_endowment_contribution":0.7806010030615194,"citer_contribution":6.681299157174008,"corpus_percentile":null,"corpus_rank":null,"citation_count":181,"citer_count":143,"citers_with_citation_signal":133,"citers_with_endowment":133,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":227795,"name":"Yang Wang","orcid":"0000-0002-5993-9368","position":1,"is_corresponding":false},{"id":862094,"name":"Maureen J. Gorman","orcid":"0000-0003-2005-6223","position":2,"is_corresponding":false},{"id":212661,"name":"Haobo Jiang","orcid":"0000-0003-1357-1315","position":3,"is_corresponding":false},{"id":374011,"name":"Michael R. Kanost","orcid":"0000-0002-6827-0061","position":4,"is_corresponding":false},{"id":1356112,"name":"Yifei Zhu","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Manduca sexta Serpin-3 Regulates Prophenoloxidase Activation in Response to Infection by Inhibiting Prophenoloxidase-activating Proteinases","abstract":"Many serine proteinase inhibitors of the serpin superfamily have evolved in vertebrates and invertebrates to regulate serine proteinase cascades that mediate the host defense responses. We have isolated an immune-responsive serpin from the tobacco hornworm, Manduca sexta. This inhibitor, M. sexta serpin-3, contains a reactive site loop strikingly similar to the proteolytic activation site in prophenoloxidase (pro-PO). Molecular cloning and sequence comparison indicate that serpin-3 is orthologous to Drosophila melanogaster serpin 27A, a regulator of melanization. M. sexta serpin-3 is constitutively present in hemolymph at a low concentration of 5-12 microg/ml and increases to 30-75 microg/ml after a microbial challenge. Recombinant serpin-3 efficiently blocks pro-PO activation in the hemolymph, and it forms SDS-stable acyl-enzyme complexes with purified pro-PO-activating proteinases (PAPs) from M. sexta. PAP-serpin-3 complexes were isolated by immunoaffinity chromatography from hemolymph activated by treatment with Micrococcus luteus. Kinetic analysis of PAP-serpin-3 association strongly suggests that serpin-3 is a physiological regulator of the pro-PO activation reaction.","is_dataset_classified":null,"base_score":5.204006687076795,"endowment":5.204006687076795,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"12966082","pmcid":null,"openalex_id":"https://openalex.org/W2051973889","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM 41247","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM 58634","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R37 GM041247","title":null}],"total_grants":3,"fwci":2.9028,"citation_percentile":0.91641612,"influential_citations":0,"citation_trend":[{"year":2012,"count":9},{"year":2013,"count":10},{"year":2014,"count":13},{"year":2015,"count":10},{"year":2016,"count":6},{"year":2017,"count":13},{"year":2018,"count":6},{"year":2019,"count":9},{"year":2020,"count":4},{"year":2021,"count":10},{"year":2022,"count":2},{"year":2023,"count":5},{"year":2024,"count":7},{"year":2025,"count":2}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://www.sciencedirect.com/science/article/pii/S002192582075934X/pdf","host_type":"journal"},{"url":"https://www.sciencedirect.com/science/article/pii/S002192582075934X/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S002192582075934X?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S002192582075934X?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.M309682200","host_type":"publisher"},{"url":"https://doi.org/10.1074/jbc.m309682200","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/12966082","host_type":"repository"}],"fields_of_study":["Invertebrate Immune Response Mechanisms","Insect symbiosis and bacterial influences","Insect Resistance and Genetics","Animals","Base Sequence","Binding Sites","Catechol Oxidase","Cloning, Molecular","Enzyme Activation","Enzyme Precursors","Hemolymph","Infections","Manduca","Molecular Sequence Data","Protease Inhibitors","Sequence Alignment","Sequence Analysis","Serpins"],"mesh_terms":["Animals","Base Sequence","Binding Sites","Cloning, Molecular","Catechol Oxidase","Enzyme Activation","Enzyme Precursors","Hemolymph","Infections","Molecular Sequence Data","Protease Inhibitors","Serpins","Sequence Alignment","Sequence Analysis","Manduca"],"keywords":["Prophenoloxidase","Serpin","Manduca sexta","Hemolymph","Biology","Serine Proteinase Inhibitors","Biochemistry","Serine protease","Serine","Proteases","Manduca","Zymogen","Molecular biology","Enzyme","Protease","Innate immune system","Insect"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T02:29:51.783342Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}