{"doi":"10.1074/jbc.m113.539890","title":"Fat-specific Protein 27 (FSP27) Interacts with Adipose Triglyceride Lipase (ATGL) to Regulate Lipolysis and Insulin Sensitivity in Human Adipocytes","abstract":null,"journal":"Journal of Biological Chemistry","year":2014,"id":637872,"datarank":0.7168685239667295,"base_score":4.77912349311153,"endowment":4.77912349311153,"self_citation_contribution":0.7168685239667295,"citation_network_contribution":0.0,"self_endowment_contribution":0.7168685239667295,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":118,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1656450,"name":"Rajween Kaur","orcid":null,"position":1,"is_corresponding":false},{"id":305691,"name":"Jun Yin","orcid":"0000-0003-4543-1229","position":2,"is_corresponding":false},{"id":1616846,"name":"Martina Schweiger","orcid":null,"position":3,"is_corresponding":false},{"id":1656451,"name":"Vishva Mitra Sharma","orcid":null,"position":4,"is_corresponding":false},{"id":1656452,"name":"Mi-Jeong Lee","orcid":null,"position":5,"is_corresponding":false},{"id":1656453,"name":"Yasuo Ido","orcid":null,"position":6,"is_corresponding":false},{"id":1656455,"name":"Cynthia M. 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In this study, we used cultured human adipocytes to demonstrate that fat-specific protein 27 (FSP27), an abundantly expressed protein in adipocytes, regulates both basal and stimulated lipolysis by interacting with adipose triglyceride lipase (ATGL, also called desnutrin or PNPLA2). We identified a core domain of FSP27, amino acids 120-220, that interacts with ATGL to inhibit its lipolytic function and promote triglyceride storage. We also defined the role of FSP27 in free fatty acid-induced insulin resistance in adipocytes. FSP27 depletion in human adipocytes increased lipolysis and inhibited insulin signaling by decreasing AKT phosphorylation. However, reducing lipolysis by either depletion of ATGL or expression of exogenous full-length FSP27 or amino acids 120-220 protected human adipocytes against the adverse effects of free fatty acids on insulin signaling. In embryonic fibroblasts derived from ATGL KO mice, exogenous free fatty acids did not affect insulin sensitivity. Our results demonstrate a crucial role for FSP27-ATGL interactions in regulating lipolysis, triglyceride accumulation, and insulin signaling in human adipocytes.","is_dataset_classified":null,"base_score":4.77912349311153,"endowment":4.77912349311153,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24627478","pmcid":"PMC4002109","openalex_id":"https://openalex.org/W2030251861","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"R56DK094815","title":null},{"funder_name":"National Institutes of Health","grant_id":"P30DK046200","title":null},{"funder_name":"National Institutes of Health","grant_id":"8KL2TR000158","title":null},{"funder_name":"Austrian Science Fund FWF","grant_id":"P 25193","title":null},{"funder_name":"NCATS NIH HHS","grant_id":"UL1-TR000157","title":null},{"funder_name":"Austrian Science Fund FWF","grant_id":"Z 136","title":null},{"funder_name":"Austrian Science Fund FWF","grant_id":"F 3002","title":null},{"funder_name":"NCATS NIH HHS","grant_id":"KL2 TR000158","title":null},{"funder_name":"Austrian Science Fund FWF","grant_id":"F 3001","title":null},{"funder_name":"NCATS NIH HHS","grant_id":"UL1 TR000157","title":null}],"total_grants":10,"fwci":6.9459,"citation_percentile":0.97366741,"influential_citations":0,"citation_trend":[{"year":2014,"count":9},{"year":2015,"count":9},{"year":2016,"count":10},{"year":2017,"count":11},{"year":2018,"count":9},{"year":2019,"count":19},{"year":2020,"count":11},{"year":2021,"count":5},{"year":2022,"count":14},{"year":2023,"count":5},{"year":2024,"count":10},{"year":2025,"count":4},{"year":2026,"count":2}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.jbc.org/article/S0021925820415194/pdf","host_type":"journal"},{"url":"http://www.jbc.org/article/S0021925820415194/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820415194?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820415194?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.M113.539890","host_type":"publisher"},{"url":"https://doi.org/10.1074/jbc.m113.539890","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24627478","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4002109","host_type":"repository"},{"url":"https://lup.lub.lu.se/record/5425013","host_type":"repository"}],"fields_of_study":["Lipid metabolism and biosynthesis","Adipose Tissue and Metabolism","Endoplasmic Reticulum Stress and Disease","3T3-L1 Cells","Adipocytes","Animals","Apoptosis Regulatory Proteins","Humans","Insulin","Insulin Resistance","Lipase","Lipolysis","Mice","Mice, Knockout","Phosphorylation","Protein Binding","Proteins","Signal Transduction","Triglycerides"],"mesh_terms":["Animals","Humans","Insulin","Insulin Resistance","Lipase","Lipolysis","Phosphorylation","Protein Binding","Proteins","Triglycerides","Signal Transduction","Adipocytes","Mice, Knockout","3T3-L1 Cells","Apoptosis Regulatory Proteins","Mice"],"keywords":["Adipose triglyceride lipase","Lipolysis","Perilipin","Lipid droplet","Adipose tissue","Internal medicine","Triglyceride","Endocrinology","Lipase","Hormone-sensitive lipase","Chemistry","Biology","Biochemistry","Enzyme","Cholesterol","Medicine","Obesity","Fatty acid","Diacylglycerol","Diabetes","Insulin resistance","Adipocyte","Fatty acid metabolism","Adipose Tissue Metabolism"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T19:40:22.486102Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}