{"doi":"10.1074/jbc.m110.192518","title":"The RNA Binding Motif Protein 15B (RBM15B/OTT3) Is a Functional Competitor of Serine-Arginine (SR) Proteins and Antagonizes the Positive Effect of the CDK11p110-Cyclin L2α Complex on Splicing","abstract":null,"journal":"Journal of Biological Chemistry","year":2011,"id":593110,"datarank":1.1687645630489272,"base_score":3.258096538021482,"endowment":3.258096538021482,"self_citation_contribution":0.4887144807032224,"citation_network_contribution":0.6800500823457049,"self_endowment_contribution":0.4887144807032224,"citer_contribution":0.6800500823457049,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":22,"citers_with_citation_signal":18,"citers_with_endowment":18,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1517969,"name":"Adeline Busson","orcid":null,"position":1,"is_corresponding":false},{"id":864384,"name":"Janeen H. Trembley","orcid":"0000-0003-3597-2611","position":2,"is_corresponding":false},{"id":652298,"name":"Judith Hyle","orcid":null,"position":3,"is_corresponding":false},{"id":1517970,"name":"Jose Grenet","orcid":null,"position":4,"is_corresponding":false},{"id":525139,"name":"Wei Zhao","orcid":"0000-0001-9437-3198","position":5,"is_corresponding":false},{"id":1517971,"name":"Catherine Ribault","orcid":null,"position":6,"is_corresponding":false},{"id":1517972,"name":"Tristan Montier","orcid":null,"position":7,"is_corresponding":false},{"id":1517973,"name":"Vincent J. Kidd","orcid":null,"position":8,"is_corresponding":false},{"id":1517974,"name":"Jill M. Lahti","orcid":null,"position":9,"is_corresponding":false},{"id":1517968,"name":"Pascal Loyer","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The RNA Binding Motif Protein 15B (RBM15B/OTT3) Is a Functional Competitor of Serine-Arginine (SR) Proteins and Antagonizes the Positive Effect of the CDK11p110-Cyclin L2α Complex on Splicing","abstract":"Here, we report the identification of the RNA binding motif protein RBM15B/OTT3 as a new CDK11(p110) binding partner that alters the effects of CDK11 on splicing. RBM15B was initially identified as a binding partner of the Epstein-Barr virus mRNA export factor and, more recently, as a cofactor of the nuclear export receptor NXF1. In this study, we found that RBM15B co-elutes with CDK11(p110), cyclin L2α, and serine-arginine (SR) proteins, including SF2/ASF, in a large nuclear complex of ∼1-MDa molecular mass following size exclusion chromatography. Using co-immunoprecipitation experiments and in vitro pulldown assays, we mapped two distinct domains of RBM15B that are essential for its direct interaction with the N-terminal extension of CDK11(p110), cyclin L2α, and SR proteins such as 9G8 and SF2/ASF. Finally, we established that RBM15B is a functional competitor of the SR proteins SF2/ASF and 9G8, inhibits formation of the functional spliceosomal E complex, and antagonizes the positive effect of the CDK11(p110)-cyclin L2α complex on splicing both in vitro and in vivo.","is_dataset_classified":null,"base_score":3.258096538021482,"endowment":3.258096538021482,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21044963","pmcid":"PMC3012969","openalex_id":"https://openalex.org/W1976776005","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"P30 CA021765","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01GM044088","title":null}],"total_grants":2,"fwci":0.3732,"citation_percentile":0.58574502,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1},{"year":2014,"count":1},{"year":2015,"count":3},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2019,"count":2},{"year":2022,"count":5},{"year":2023,"count":4},{"year":2024,"count":2},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.jbc.org/article/S0021925820542051/pdf","host_type":"journal"},{"url":"http://www.jbc.org/article/S0021925820542051/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820542051?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820542051?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.M110.192518","host_type":"publisher"},{"url":"https://doi.org/10.1074/jbc.m110.192518","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21044963","host_type":"repository"},{"url":"https://hal.science/hal-00739976","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3012969","host_type":"repository"}],"fields_of_study":["RNA Research and Splicing","RNA modifications and cancer","Nuclear Structure and Function"],"mesh_terms":["Serine-Arginine Splicing Factors","Animals","Binding, Competitive","Cell Nucleus","Humans","Nuclear Proteins","RNA Splicing","RNA, Messenger","Cyclins","RNA-Binding Proteins","Spliceosomes","Protein Structure, Tertiary","Cyclin-Dependent Kinases","Protein Transport","Nucleocytoplasmic Transport Proteins","HEK293 Cells"],"keywords":["SR protein","RNA-binding protein","RNA splicing","Biology","Immunoprecipitation","Cell biology","Splicing factor","Nuclear export signal","Heterogeneous ribonucleoprotein particle","RNA","Molecular biology","Biochemistry","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Reduced inequalities"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-26T17:15:12.151336Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}