{"doi":"10.1074/jbc.m110.158634","title":"Rabconnectin-3 Is a Functional Regulator of Mammalian Notch Signaling","abstract":null,"journal":"Journal of Biological Chemistry","year":2010,"id":660238,"datarank":0.6456097639806255,"base_score":4.304065093204169,"endowment":4.304065093204169,"self_citation_contribution":0.6456097639806255,"citation_network_contribution":0.0,"self_endowment_contribution":0.6456097639806255,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":73,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":994259,"name":"Yan Yan","orcid":"0000-0002-6344-136X","position":1,"is_corresponding":false},{"id":1723520,"name":"Debra Quek","orcid":null,"position":2,"is_corresponding":false},{"id":1723522,"name":"Trudi Schupbach","orcid":null,"position":3,"is_corresponding":false},{"id":395559,"name":"Yibin Kang","orcid":"0000-0002-1626-6730","position":4,"is_corresponding":false},{"id":1723518,"name":"Nilay Sethi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Rabconnectin-3 Is a Functional Regulator of Mammalian Notch Signaling","abstract":"The Notch signaling pathway is important for cell fate decisions in embryonic development and adult life. Defining the functional importance of the Notch pathway in these contexts requires the elucidation of essential signal transduction components that have not been fully characterized. Here, we show that Rabconnectin-3B is required for the Notch pathway in mammalian cells. siRNA-mediated silencing of Rabconnectin-3B in mammalian cells attenuated Notch signaling and disrupted the activation and nuclear accumulation of the Notch target Hes1. Rabconnectin-3B knockdown also disrupted V-ATPase activity in mammalian cells, consistent with previous observations in Drosophila. Pharmacological inhibition of the V-ATPase complex significantly reduced Notch signaling in mammalian cells. Finally, Rabconnectin-3B knockdown phenocopied functional disruption of Notch signaling during osteoclast differentiation. Collectively, these findings define an important role for Rabconnectin-3 and V-ATPase activity in the Notch signaling pathway in mammalian cells.","is_dataset_classified":null,"base_score":4.304065093204169,"endowment":4.304065093204169,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20810660","pmcid":"PMC2966091","openalex_id":"https://openalex.org/W1990534862","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"R01 CA134519","title":null},{"funder_name":"NCI NIH HHS","grant_id":"BC051647","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM077620","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01GM077620-08","title":"EGF receptor mediated signaling in Drosophila"},{"funder_name":"National Institutes of Health","grant_id":"1R01CA134519-01","title":"Metadherin in Metastasis and Chemoresistance of Breast Cancer"},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null}],"total_grants":6,"fwci":1.37,"citation_percentile":0.78948513,"influential_citations":0,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":5},{"year":2014,"count":9},{"year":2015,"count":14},{"year":2016,"count":4},{"year":2017,"count":4},{"year":2018,"count":1},{"year":2019,"count":8},{"year":2020,"count":5},{"year":2021,"count":4},{"year":2022,"count":3},{"year":2023,"count":4},{"year":2024,"count":2},{"year":2025,"count":2},{"year":2026,"count":2}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.jbc.org/article/S0021925820470176/pdf","host_type":"journal"},{"url":"http://www.jbc.org/article/S0021925820470176/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820470176?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820470176?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.M110.158634","host_type":"publisher"},{"url":"https://doi.org/10.1074/jbc.m110.158634","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20810660","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2966091","host_type":"repository"},{"url":"http://repository.hkust.edu.hk/ir/Record/1783.1-52376","host_type":"repository"},{"url":"http://www.jbc.org/content/285/45/34757.full.pdf","host_type":"Unpaywall"},{"url":"https://dx.doi.org/10.1074/jbc.m110.158634","host_type":""},{"url":"https://doi.org/https://doi.org/10.1074/jbc.M110.158634","host_type":""}],"fields_of_study":["Developmental Biology and Gene Regulation","Marine Ecology and Invasive Species","Bone Metabolism and Diseases","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Transcription Factor HES-1","Animals","Cell Differentiation","Drosophila melanogaster","Humans","Nerve Tissue Proteins","Osteoclasts","Signal Transduction","Homeodomain Proteins","Vacuolar Proton-Translocating ATPases","RNA, Small Interfering","Cell Line, Tumor","Adaptor Proteins, Signal Transducing","Mice","Basic Helix-Loop-Helix Proteins","Basic Helix-Loop-Helix Transcription Factors","Receptors, Notch","Gene Knockdown Techniques"],"keywords":["Notch signaling pathway","Hes3 signaling axis","Cell biology","HES1","Signal transduction","Notch proteins","Gene knockdown","Biology","Regulator","Cell fate determination","Cyclin-dependent kinase 8","Cell signaling","Transcription factor","Cell culture","Genetics","Gene","Basic Helix-Loop-Helix Proteins","Homeodomain Proteins","570","Vacuolar Proton-Translocating ATPases","Receptors, Notch","Osteoclasts","Cell Differentiation","Nerve Tissue Proteins","Mice","Drosophila melanogaster","Cell Line, Tumor","Gene Knockdown Techniques","Animals","Humans","Transcription Factor HES-1","RNA, Small Interfering","Adaptor Proteins, Signal Transducing"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T08:46:28.733145Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}