{"doi":"10.1074/jbc.m109.052126","title":"Tobacco Calcium-dependent Protein Kinases Are Differentially Phosphorylated in Vivo as Part of a Kinase Cascade That Regulates Stress Response","abstract":null,"journal":"Journal of Biological Chemistry","year":2010,"id":676698,"datarank":0.6766289259775276,"base_score":4.51085950651685,"endowment":4.51085950651685,"self_citation_contribution":0.6766289259775276,"citation_network_contribution":0.0,"self_endowment_contribution":0.6766289259775276,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":90,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1768077,"name":"Nana Keinath","orcid":null,"position":1,"is_corresponding":false},{"id":1768078,"name":"Ullrich Dubiella","orcid":null,"position":2,"is_corresponding":false},{"id":1768079,"name":"Raphael Demoulière","orcid":null,"position":3,"is_corresponding":false},{"id":1768080,"name":"Anindita Seal","orcid":null,"position":4,"is_corresponding":false},{"id":1768081,"name":"Tina Romeis","orcid":null,"position":5,"is_corresponding":false},{"id":1768076,"name":"Claus-Peter Witte","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Tobacco Calcium-dependent Protein Kinases Are Differentially Phosphorylated in Vivo as Part of a Kinase Cascade That Regulates Stress Response","abstract":"In vivo phosphorylation sites of the tobacco calcium-dependent protein kinases NtCDPK2 and NtCDPK3 were determined in response to biotic or abiotic stress. Stress-inducible phosphorylation was exclusively located in the variable N termini, where both kinases were phosphorylated differentially despite 91% overall sequence identity. In NtCDPK2, serine 40 and threonine 65 were phosphorylated within 2 min after stress. Whereas Thr(65) is subjected to intra-molecular in vivo autophosphorylation, Ser(40) represents a target for a regulatory upstream protein kinase, and correct NtCDPK2 membrane localization is required for Ser(40) phosphorylation. NtCDPK3 is phosphorylated at least at two sites in the N terminus by upstream kinase(s) upon stress stimulus, first at Ser(54), a site not present in NtCDPK2, and also at a second undetermined site not identical to Ser(40). Domain swap experiments established that differential phosphorylation of both kinases is exclusively determined by the respective N termini. A cell death-inducing response was only observed upon expression of a truncated variant lacking the junction and calcium-binding domain of NtCDPK2 (VK2). This response required protein kinase activity and was reduced when subcellular membrane localization was disturbed by a mutation in the myristoylation and palmitoylation site. Our data indicate that CDPKs are integrated in stress-dependent protein kinase signaling cascades, and regulation of CDPK function in response to in vivo stimulation is dependent on its membrane localization.","is_dataset_classified":null,"base_score":4.51085950651685,"endowment":4.51085950651685,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20118232","pmcid":"PMC2843223","openalex_id":"https://openalex.org/W2087122633","authors":[],"funders":[],"total_grants":0,"fwci":3.8604,"citation_percentile":0.94340044,"influential_citations":0,"citation_trend":[{"year":2012,"count":9},{"year":2013,"count":10},{"year":2014,"count":9},{"year":2015,"count":4},{"year":2016,"count":7},{"year":2017,"count":8},{"year":2018,"count":6},{"year":2019,"count":7},{"year":2020,"count":4},{"year":2021,"count":2},{"year":2022,"count":5},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":2},{"year":2026,"count":3}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.jbc.org/article/S002192581955030X/pdf","host_type":"journal"},{"url":"http://www.jbc.org/article/S002192581955030X/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S002192581955030X?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S002192581955030X?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.M109.052126","host_type":"publisher"},{"url":"https://doi.org/10.1074/jbc.m109.052126","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20118232","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2843223","host_type":"repository"},{"url":"http://edoc.mpg.de/476913","host_type":"repository"},{"url":"http://hdl.handle.net/11858/00-001M-0000-0012-354C-9","host_type":"repository"}],"fields_of_study":["Fungal and yeast genetics research","Plant Stress Responses and Tolerance","Plant Gene Expression Analysis"],"mesh_terms":["Amino Acid Sequence","Calcium","Kinetics","Molecular Sequence Data","Phenotype","Phosphorylation","Protein Kinases","Mass Spectrometry","Nicotiana","Signal Transduction","Gene Expression Regulation, Enzymologic","Mutagenesis, Site-Directed","Arabidopsis","Sequence Homology, Amino Acid","Calcium-Calmodulin-Dependent Protein Kinases","Gene Expression Regulation, Plant"],"keywords":["Autophosphorylation","Phosphorylation","Kinase","Phosphorylation cascade","Cell biology","Biology","Protein phosphorylation","Protein kinase A","MAP2K7","Protein kinase domain","MAPK14","MAP kinase kinase kinase","Biochemistry","Chemistry","Cyclin-dependent kinase 2","Mutant"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T02:59:03.091593Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}