{"doi":"10.1074/jbc.m001160200","title":"RalGEF2, a Pleckstrin Homology Domain Containing Guanine Nucleotide Exchange Factor for Ral","abstract":null,"journal":"Journal of Biological Chemistry","year":2000,"id":619456,"datarank":0.586803450814222,"base_score":3.912023005428146,"endowment":3.912023005428146,"self_citation_contribution":0.586803450814222,"citation_network_contribution":0.0,"self_endowment_contribution":0.586803450814222,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":49,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":589825,"name":"Johan de Rooij","orcid":"0000-0001-6101-7875","position":1,"is_corresponding":false},{"id":1598580,"name":"Rob M.F. Wolthuis","orcid":null,"position":2,"is_corresponding":false},{"id":592932,"name":"Holger Rehmann","orcid":null,"position":3,"is_corresponding":false},{"id":1598581,"name":"Joep Wesenbeek","orcid":null,"position":4,"is_corresponding":false},{"id":1598582,"name":"Robbert H. Cool","orcid":null,"position":5,"is_corresponding":false},{"id":1598584,"name":"Alfred H. Wittinghofer","orcid":null,"position":6,"is_corresponding":false},{"id":1598586,"name":"Johannes L. Bos","orcid":null,"position":7,"is_corresponding":false},{"id":1598579,"name":"Kim M.T. de Bruyn","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"RalGEF2, a Pleckstrin Homology Domain Containing Guanine Nucleotide Exchange Factor for Ral","abstract":"Ral is a ubiquitously expressed Ras-like small GTPase. Several guanine nucleotide exchange factors for Ral have been identified, including members of the RalGDS family, which exhibit a Ras binding domain and are regulated by binding to RasGTP. Here we describe a novel type of RalGEF, RalGEF2. This guanine nucleotide exchange factor has a characteristic Cdc25-like catalytic domain at the N terminus and a pleckstrin homology (PH) domain at the C terminus. RalGEF2 is able to activate Ral both in vivo and in vitro. Deletion of the PH domain results in an increased cytoplasmic localization of the protein and a corresponding reduction in activity in vivo, suggesting that the PH domain functions as a membrane anchor necessary for optimal activity in vivo.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10889189","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.jbc.org/article/S0021925819794654/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925819794654?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925819794654?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.M001160200","host_type":"publisher"}],"fields_of_study":["Amino Acid Sequence","Animals","Blood Proteins","COS Cells","Enzyme Activation","Molecular Sequence Data","Phosphoproteins","Sequence Alignment","Substrate Specificity","ral GTP-Binding Proteins","ral Guanine Nucleotide Exchange Factor"],"mesh_terms":["COS Cells","Animals","ral GTP-Binding Proteins","ral Guanine Nucleotide Exchange Factor","Blood Proteins","Phosphoproteins","Sequence Alignment","Enzyme Activation","Amino Acid Sequence","Substrate Specificity","Molecular Sequence Data"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T07:15:45.926906Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}