{"doi":"10.1074/jbc.c400373200","title":"Heparan Sulfate Synthesized by Mouse Embryonic Stem Cells Deficient in NDST1 and NDST2 Is 6-O-Sulfated but Contains No N-Sulfate Groups","abstract":null,"journal":"Journal of Biological Chemistry","year":2004,"id":609610,"datarank":0.687745121800586,"base_score":4.584967478670572,"endowment":4.584967478670572,"self_citation_contribution":0.687745121800586,"citation_network_contribution":0.0,"self_endowment_contribution":0.687745121800586,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":97,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":959603,"name":"Johan Ledin","orcid":"0000-0002-7319-7735","position":1,"is_corresponding":false},{"id":1566958,"name":"Emanuel Smeds","orcid":null,"position":2,"is_corresponding":false},{"id":1566959,"name":"Inger Eriksson","orcid":null,"position":3,"is_corresponding":false},{"id":1566960,"name":"Marion Kusche-Gullberg","orcid":null,"position":4,"is_corresponding":false},{"id":959602,"name":"Lena Kjellén","orcid":"0000-0003-3973-918X","position":5,"is_corresponding":false},{"id":1566957,"name":"Katarina Holmborn","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Heparan Sulfate Synthesized by Mouse Embryonic Stem Cells Deficient in NDST1 and NDST2 Is 6-O-Sulfated but Contains No N-Sulfate Groups","abstract":"Heparan sulfate structure differs significantly between various cell types and during different developmental stages. The diversity is created during biosynthesis by sulfotransferases, which add sulfate groups to the growing chain, and a C5-epimerase, which converts selected glucuronic acid residues to iduronic acid. All these modifications are believed to depend on initial glucosamine N-sulfation carried out by the enzyme glucosaminyl N-deacetylase/N-sulfotransferase (NDST). Here we report that heparan sulfate synthesized by mouse embryonic stem cells deficient in NDST1 and NDST2 completely lacks N-sulfation but still contains 6-O-sulfate groups, demonstrating that 6-O-sulfation can occur without prior N-sulfation. Reverse transcriptase-PCR analysis indicates that all three identified 6-O-sulfotransferases are expressed by the cells, 6-O-sulfotransferase-1 being the dominating form. The 6-O-sulfated polysaccharide lacking N-sulfate groups also contains N-unsubstituted glucosamine units, raising questions about how these units are generated.","is_dataset_classified":null,"base_score":4.584967478670572,"endowment":4.584967478670572,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15319440","pmcid":null,"openalex_id":"https://openalex.org/W2070318120","authors":[],"funders":[],"total_grants":0,"fwci":2.5517,"citation_percentile":0.88514357,"influential_citations":0,"citation_trend":[{"year":2012,"count":9},{"year":2013,"count":6},{"year":2014,"count":9},{"year":2015,"count":1},{"year":2016,"count":9},{"year":2017,"count":2},{"year":2018,"count":4},{"year":2019,"count":1},{"year":2020,"count":4},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1074/jbc.c400373200","host_type":"journal"},{"url":"https://doi.org/10.1074/jbc.c400373200","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820769747?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925820769747?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.C400373200","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15319440","host_type":"repository"}],"fields_of_study":["Proteoglycans and glycosaminoglycans research","Glycosylation and Glycoproteins Research","Connective tissue disorders research"],"mesh_terms":["Amidohydrolases","Animals","Blastocyst","Carbohydrate Epimerases","Chromatography, High Pressure Liquid","Chromatography, Ion Exchange","Embryo, Mammalian","Genotype","Glucosamine","Glycosaminoglycans","Heparitin Sulfate","Heparan Sulfate","Iduronic Acid","Mice, Transgenic","Nitrous Acid","Polysaccharides","Stem Cells","Sulfates","Sulfur","Sulfotransferases","DNA, Complementary","Gene Expression Regulation, Developmental","Reverse Transcriptase Polymerase Chain Reaction","Glucuronic Acid","Mice"],"keywords":["Sulfation","Heparan sulfate","Iduronic acid","Glucosamine","Biochemistry","Glucuronic acid","Chemistry","Sulfate","Sulfotransferase","Embryonic stem cell","Enzyme","Perlecan","Glycosaminoglycan","Polysaccharide","Gene","Organic chemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-31T10:55:00.131129Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}