{"doi":"10.1074/jbc.274.49.34527","title":"Cyclin K Functions as a CDK9 Regulatory Subunit and Participates in RNA Polymerase II Transcription","abstract":null,"journal":"Journal of Biological Chemistry","year":1999,"id":616213,"datarank":0.811075765719018,"base_score":5.407171771460119,"endowment":5.407171771460119,"self_citation_contribution":0.811075765719018,"citation_network_contribution":0.0,"self_endowment_contribution":0.811075765719018,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":222,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":97162,"name":"Junmin Peng","orcid":"0000-0003-0472-7648","position":1,"is_corresponding":false},{"id":638024,"name":"Gary Lee","orcid":"0000-0002-0036-511X","position":2,"is_corresponding":false},{"id":353197,"name":"David H. Price","orcid":"0000-0002-5597-385X","position":3,"is_corresponding":false},{"id":1588619,"name":"Osvaldo Flores","orcid":null,"position":4,"is_corresponding":false},{"id":1588614,"name":"Tsu-Ju Fu","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Cyclin K Functions as a CDK9 Regulatory Subunit and Participates in RNA Polymerase II Transcription","abstract":"Important progress in the understanding of elongation control by RNA polymerase II (RNAPII) has come from the recent identification of the positive transcription elongation factor b (P-TEFb) and the demonstration that this factor is a protein kinase that phosphorylates the carboxyl-terminal domain (CTD) of the RNAPII largest subunit. The P-TEFb complex isolated from mammalian cells contains a catalytic subunit (CDK9), a cyclin subunit (cyclin T1 or cyclin T2), and additional, yet unidentified, polypeptides of unknown function. To identify additional factors involved in P-TEFb function we performed a yeast two-hybrid screen using CDK9 as bait and found that cyclin K interacts with CDK9 in vivo. Biochemical analyses indicate that cyclin K functions as a regulatory subunit of CDK9. The CDK9-cyclin K complex phosphorylated the CTD of RNAPII and functionally substituted for P-TEFb comprised of CDK9 and cyclin T in in vitro transcription reactions.","is_dataset_classified":null,"base_score":5.407171771460119,"endowment":5.407171771460119,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10574912","pmcid":null,"openalex_id":"https://openalex.org/W2079489048","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"AI46391","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM 35500","title":null}],"total_grants":2,"fwci":4.8787,"citation_percentile":0.95912876,"influential_citations":0,"citation_trend":[{"year":2012,"count":11},{"year":2013,"count":9},{"year":2014,"count":9},{"year":2015,"count":9},{"year":2016,"count":13},{"year":2017,"count":1},{"year":2018,"count":4},{"year":2019,"count":7},{"year":2020,"count":6},{"year":2021,"count":3},{"year":2022,"count":6},{"year":2023,"count":9},{"year":2024,"count":6},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1074/jbc.274.49.34527","host_type":"journal"},{"url":"https://doi.org/10.1074/jbc.274.49.34527","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925819533555?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925819533555?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.274.49.34527","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10574912","host_type":"repository"},{"url":"http://www.jbc.org/content/274/49/34527.full.pdf","host_type":"Unpaywall"}],"fields_of_study":["RNA Research and Splicing","Genomics and Chromatin Dynamics","RNA modifications and cancer","Chromatography, Gel","Cyclin-Dependent Kinase 9","Cyclin-Dependent Kinases","Cyclins","Dose-Response Relationship, Drug","Gene Expression Regulation, Enzymologic","HeLa Cells","Humans","Kinetics","Protein Binding","RNA Polymerase II","Transcription, Genetic","Two-Hybrid System Techniques"],"mesh_terms":["Chromatography, Gel","Dose-Response Relationship, Drug","HeLa Cells","Humans","Kinetics","Protein Binding","RNA Polymerase II","Transcription, Genetic","Gene Expression Regulation, Enzymologic","Cyclins","Cyclin-Dependent Kinases","Two-Hybrid System Techniques","Cyclin-Dependent Kinase 9","Hela Cells"],"keywords":["P-TEFb","RNA polymerase II","Cyclin-dependent kinase complex","Cyclin-dependent kinase 9","Cyclin A","Protein subunit","Molecular biology","Cyclin D","Cyclin A2","Cyclin-dependent kinase","Biology","Cell biology","Cyclin","Transcription (linguistics)","Kinase","Cyclin-dependent kinase 2","Protein kinase A","Biochemistry","Cell cycle","Gene expression","Promoter","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T22:18:47.176574Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}