{"doi":"10.1074/jbc.274.42.30310","title":"Stimulation of DNA Replication in Saccharomyces cerevisiae by a Glutamine- and Proline-rich Transcriptional Activation Domain","abstract":null,"journal":"Journal of Biological Chemistry","year":1999,"id":655633,"datarank":0.44166584687496613,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.0,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1254571,"name":"Rong Li","orcid":"0000-0001-7266-0241","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Stimulation of DNA Replication in Saccharomyces cerevisiae by a Glutamine- and Proline-rich Transcriptional Activation Domain","abstract":"Glutamine-rich Sp1 and proline-rich CTF1, two extensively studied mammalian transcription factors, bind to origins of replication in DNA tumor viruses and stimulate viral DNA replication in mammalian cells. Here it is shown that, when tethered to a plasmid-borne cellular origin of replication, the activation domains of both proteins can enhance origin function in Saccharomyces cerevisiae. Hydrophobic patches in Sp1 and CTF1 that mediate transcriptional activation in higher eukaryotes are also important for activation of replication in yeast. However, only the activation domain of CTF1 can enhance initiation of replication from a chromosomally embedded origin. This correlates with the ability of CTF1 to alter the local chromatin structure around the chromosomal origin of replication. The CTF1-induced chromatin remodeling occurs at multiple stages of the cell cycle. These findings strongly suggest a high degree of conservation in the mechanisms used by various types of transcription factors to stimulate viral and cellular DNA replication in eukaryotes.","is_dataset_classified":null,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10514526","pmcid":null,"openalex_id":"https://openalex.org/W2034939127","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM57893","title":null}],"total_grants":1,"fwci":1.1342,"citation_percentile":0.76607354,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2017,"count":2},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1074/jbc.274.42.30310","host_type":"journal"},{"url":"https://doi.org/10.1074/jbc.274.42.30310","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925819518993?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925819518993?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.274.42.30310","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10514526","host_type":"repository"},{"url":"http://www.jbc.org/content/274/42/30310.full.pdf","host_type":"Unpaywall"}],"fields_of_study":["Genomics and Chromatin Dynamics","DNA Repair Mechanisms","Epigenetics and DNA Methylation"],"mesh_terms":["Amino Acid Sequence","Chromatin","Chromosomal Proteins, Non-Histone","DNA Replication","DNA-Binding Proteins","DNA, Fungal","Fungal Proteins","Glutamine","Plasmids","Proline","Protein Conformation","Saccharomyces cerevisiae","Transcription Factors","Transcriptional Activation","Chromosomes, Fungal","Sp1 Transcription Factor","Replication Origin","Saccharomyces cerevisiae Proteins"],"keywords":["Origin recognition complex","Control of chromosome duplication","DNA replication factor CDT1","Pre-replication complex","Licensing factor","Eukaryotic DNA replication","Biology","Minichromosome maintenance","Origin of replication","Replication factor C","DNA replication","Saccharomyces cerevisiae","Chromatin","Cell biology","Transcription (linguistics)","DNA","Genetics","Yeast"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-11T18:22:32.714170Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}