{"doi":"10.1074/jbc.274.1.368","title":"Functional β1-Integrins Release the Suppression of Fibronectin Matrix Assembly by Vitronectin","abstract":null,"journal":"Journal of Biological Chemistry","year":1999,"id":680171,"datarank":0.4636563680037475,"base_score":3.091042453358316,"endowment":3.091042453358316,"self_citation_contribution":0.4636563680037475,"citation_network_contribution":0.0,"self_endowment_contribution":0.4636563680037475,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":675534,"name":"Takao Sakai","orcid":"0000-0002-7375-5450","position":1,"is_corresponding":false},{"id":1777111,"name":"Julie Nowlen","orcid":null,"position":2,"is_corresponding":false},{"id":1638927,"name":"Izumi Hayashi","orcid":null,"position":3,"is_corresponding":false},{"id":138705,"name":"Reinhard Fässler","orcid":null,"position":4,"is_corresponding":false},{"id":109575,"name":"Deane F. Mosher","orcid":"0000-0002-1233-1005","position":5,"is_corresponding":false},{"id":1700715,"name":"Qinghong Zhang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Functional β1-Integrins Release the Suppression of Fibronectin Matrix Assembly by Vitronectin","abstract":"beta1-null GD25 fibroblasts adherent to vitronectin fail to bind the N-terminal 70-kDa matrix assembly domain of fibronectin or to assemble fibronectin (Sakai, T., Zhang, Q., Fässler, R., and Mosher, D. F. (1998) J. Cell Biol. 141, 527-538). We have made four observations that extend this finding. First, the presence of vitronectin on a substrate that otherwise can support fibronectin assembly has a dominant-negative effect on assembly. Second, the dominant-negative effect is lost when active beta1A is expressed. Third, beta1A containing the extracellular D130A inactivating mutation has a dominant-negative effect on fibronectin assembly. Fourth, beta1-null cells adherent to vitronectin are flat and lack filopodia, whereas beta1-null cells adherent to fibronectin or beta1A-expressing cells adherent to either vitronectin or fibronectin are contracted and exhibit numerous filopodia. These results reveal, therefore, that GD25 cells adherent to vitronectin can only assume a shape suitable for assembly of fibronectin when there is a countervailing signal from functional beta1-integrins.","is_dataset_classified":null,"base_score":3.091042453358316,"endowment":3.091042453358316,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9867852","pmcid":null,"openalex_id":"https://openalex.org/W2038445932","authors":[],"funders":[{"funder_name":"NHLBI NIH HHS","grant_id":"HL21644","title":null}],"total_grants":1,"fwci":0.6269,"citation_percentile":0.60902068,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2015,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.jbc.org/article/S0021925818370947/pdf","host_type":"journal"},{"url":"http://www.jbc.org/article/S0021925818370947/pdf","host_type":"HYBRID"},{"url":"http://www.jbc.org/article/S0021925818370947/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925818370947?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925818370947?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.274.1.368","host_type":"publisher"},{"url":"https://doi.org/10.1074/jbc.274.1.368","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/9867852","host_type":"repository"}],"fields_of_study":["Cell Adhesion Molecules Research","Cellular Mechanics and Interactions","Protease and Inhibitor Mechanisms","Biology","Medicine"],"mesh_terms":["Amino Acid Sequence","Cells, Cultured","Extracellular Matrix","Fibronectins","Humans","Reproducibility of Results","Signal Transduction","Mutagenesis, Site-Directed","Integrin beta1","Vitronectin"],"keywords":["Vitronectin","Fibronectin","Integrin","Filopodia","Extracellular matrix","Cell biology","Matrix (chemical analysis)","Biology","Molecular biology","Chemistry","Biophysics","Biochemistry","Cell","Actin"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T14:34:10.959036Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}