{"doi":"10.1074/jbc.272.3.2005","title":"'Oxidation Inhibits Substrate Proteolysis by Calpain I but Not Autolysis","abstract":null,"journal":"Journal of Biological Chemistry","year":1997,"id":678066,"datarank":0.7168685239667295,"base_score":4.77912349311153,"endowment":4.77912349311153,"self_citation_contribution":0.7168685239667295,"citation_network_contribution":0.0,"self_endowment_contribution":0.7168685239667295,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":118,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1771659,"name":"John S. Elce","orcid":null,"position":1,"is_corresponding":false},{"id":489335,"name":"P. Darwin Bell","orcid":null,"position":2,"is_corresponding":false},{"id":1771661,"name":"J. Clay Isbell","orcid":null,"position":3,"is_corresponding":false},{"id":317968,"name":"Gail V.W. Johnson","orcid":"0000-0003-3464-0404","position":4,"is_corresponding":false},{"id":1771658,"name":"Rodney P. Guttmann","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"'Oxidation Inhibits Substrate Proteolysis by Calpain I but Not Autolysis","abstract":"In this study, the effects of oxidation on calpain I autolysis and calpain-mediated proteolysis were examined. Calpain I was incubated with increasing concentrations of free calcium in the presence or absence of oxidant, and autolytic conversion of both the 80- and 30-kDa subunits was measured by immunoblotting utilizing monoclonal antibodies which recognize both autolyzed and non-autolyzed forms of each subunit, respectively. Autolytic conversion of the 80-kDa subunit of calpain I was not detected until free calcium concentration was greater than 40 microM, whereas autolysis of the 30-kDa subunit did not occur until the free calcium concentration was greater than 100 microM. In addition, autolytic conversion of either the 80- or 30-kDa subunit was not inhibited by the presence of oxidant. Calpain I activity was measured using the fluorescent peptide N-succinyl-L-leucyl-L-leucyl-L-valyl-L-tyrosine-7-amido-4- methylcoumarin or the microtubule-associated protein tau as substrate. Calpain I was found to have proteolytic activity at free calcium concentrations below that required for autolysis. Calpain I activity was strongly inhibited by oxidant at all calcium concentrations studied, suggesting that proteolytic activity of both the non-autolyzed 80-kDa and autolyzed 76-kDa forms was susceptible to oxidation. Interestingly, whereas oxidation did not inhibit autolytic conversion, the presence of high substrate concentrations did result in a significant reduction of autolysis without altering calpain proteolytic activity. Calpain I activity that had been inhibited by the presence of oxidant was recovered immediately by addition of the reducing agent dithiothreitol.","is_dataset_classified":null,"base_score":4.77912349311153,"endowment":4.77912349311153,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8999893","pmcid":null,"openalex_id":"https://openalex.org/W1986586932","authors":[],"funders":[{"funder_name":"NIA NIH HHS","grant_id":"AG06569","title":null},{"funder_name":"NIA NIH HHS","grant_id":"AG12396","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"NS27538","title":null}],"total_grants":3,"fwci":2.8206,"citation_percentile":0.9023744,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":6},{"year":2014,"count":1},{"year":2015,"count":3},{"year":2017,"count":2},{"year":2018,"count":3},{"year":2020,"count":4},{"year":2021,"count":1},{"year":2023,"count":2},{"year":2024,"count":2},{"year":2025,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.jbc.org/article/S0021925819675149/pdf","host_type":"journal"},{"url":"http://www.jbc.org/article/S0021925819675149/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925819675149?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925819675149?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.272.3.2005","host_type":"publisher"},{"url":"https://doi.org/10.1074/jbc.272.3.2005","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8999893","host_type":"repository"}],"fields_of_study":["Calpain Protease Function and Regulation","Venomous Animal Envenomation and Studies","Endoplasmic Reticulum Stress and Disease","Calcium","Calpain","DNA, Complementary","Dithiothreitol","Humans","Hydrolysis","Indicators and Reagents","Oxidation-Reduction","Substrate Specificity"],"mesh_terms":["Calcium","Calpain","Dithiothreitol","Humans","Hydrolysis","Indicators and Reagents","Oxidation-Reduction","Substrate Specificity","DNA, Complementary"],"keywords":["Autolysis (biology)","Proteolysis","Calpain","Chemistry","Substrate (aquarium)","Cell biology","Biochemistry","Enzyme","Biology","Ecology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T04:53:45.547847Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}