{"doi":"10.1074/jbc.270.39.22653","title":"Interleukin-3 Regulates Development of the 5-Lipoxygenase/Leukotriene C4 Synthase Pathway in Mouse Mast Cells","abstract":null,"journal":"Journal of Biological Chemistry","year":1995,"id":589998,"datarank":3.927529826058102,"base_score":4.68213122712422,"endowment":4.68213122712422,"self_citation_contribution":0.7023196840686331,"citation_network_contribution":3.225210141989469,"self_endowment_contribution":0.7023196840686331,"citer_contribution":3.225210141989469,"corpus_percentile":null,"corpus_rank":null,"citation_count":107,"citer_count":73,"citers_with_citation_signal":65,"citers_with_endowment":65,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":252896,"name":"K. Frank Austen","orcid":null,"position":1,"is_corresponding":false},{"id":55628,"name":"Clifton O. Bingham","orcid":"0000-0002-4752-5029","position":2,"is_corresponding":false},{"id":1509579,"name":"Daniel S. Friend","orcid":null,"position":3,"is_corresponding":false},{"id":1509580,"name":"John F. Penrose","orcid":null,"position":4,"is_corresponding":false},{"id":1509581,"name":"Jonathan P. Arm","orcid":null,"position":5,"is_corresponding":false},{"id":301714,"name":"Makoto Murakami","orcid":"0000-0002-4565-6017","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Interleukin-3 Regulates Development of the 5-Lipoxygenase/Leukotriene C4 Synthase Pathway in Mouse Mast Cells","abstract":"To study cytokine regulation of the 5-lipoxygenase (5-LO)/leukotriene (LT) synthase pathway we have developed mouse bone marrow-derived mast cells (BMMC) that minimally express each protein of the pathway by using a novel culture system, lacking interleukin (IL)-3. When mouse bone marrow cells were cultured for 5 weeks with 100 ng/ml c-kit ligand (KL) and 10 units/ml IL-10, a population of > 95% mast cells was obtained. These cells generated 8.3 +/- 4.5 ng of LTC4/10(6) cells and 8.1 +/- 2.4 ng of prostaglandin (PG) D2/10(6) cells after IgE-dependent activation. When these BMMC were cultured for 2-5 weeks more with 100 units/ml IL-3 in the continued presence of KL and IL-10, the IgE-dependent generation of LTC4 and PGD2 increased to 212 +/- 36 and 25.5 +/- 8.6 ng/10(6) cells, respectively. The dramatic increase in the IgE-dependent generation of LTC4 in response to IL-3 was accompanied by a concomitant increase in expression of 5-LO and 5-LO-activating protein and preceded the increased expression of cytosolic phospholipase A2 and LTC4 synthase. The recognition that IL-3 up-regulates the expression of each protein of the 5-LO pathway for the generation of LTC4 contrasts with our recent finding that KL up-regulates the expression of cytosolic phospholipase A2, prostaglandin endoperoxide synthase-1, and hematopoietic PGD2 synthase and increases the IgE-dependent generation of PGD2 in BMMC developed from bone marrow with IL-3. Thus, developmentally segregated regulation of the prostanoid and cysteinyl leukotriene pathways in lineage-related committed mast cell progenitors reveals the pleiotropism of this effector cell of allergic inflammation, a cytokine/growth factor basis for preferential expression of pathways of eicosanoid biosynthesis, and the particular role of IL-3 in regulating the expression of the proteins of the 5-LO/LTC4 synthase pathway.","is_dataset_classified":null,"base_score":4.68213122712422,"endowment":4.68213122712422,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7559381","pmcid":null,"openalex_id":"https://openalex.org/W2120356456","authors":[],"funders":[{"funder_name":"NHLBI NIH HHS","grant_id":"HL36110","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI22531","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI31599","title":null}],"total_grants":3,"fwci":2.8756,"citation_percentile":0.91848016,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":1},{"year":2014,"count":2},{"year":2015,"count":3},{"year":2017,"count":2},{"year":2018,"count":1},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1074/jbc.270.39.22653","host_type":"journal"},{"url":"https://doi.org/10.1074/jbc.270.39.22653","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925818900037?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925818900037?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/jbc.270.39.22653","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7559381","host_type":"repository"}],"fields_of_study":["Mast cells and histamine","Urticaria and Related Conditions","Drug-Induced Adverse Reactions","Animals","Arachidonate 5-Lipoxygenase","Bone Marrow Cells","Cells, Cultured","Gene Expression","Glutathione Transferase","Growth Substances","Hematopoietic Stem Cells","Humans","Immunoglobulin E","Interleukin-3","Kinetics","Leukotriene C4","Mast Cells","Mice","Phospholipases A","Phospholipases A2","Prostaglandin D2","Prostaglandin-Endoperoxide Synthases","Recombinant Proteins","Stem Cell Factor","Time Factors"],"mesh_terms":["Animals","Arachidonate 5-Lipoxygenase","Bone Marrow Cells","Cells, Cultured","Glutathione Transferase","Growth Substances","Hematopoietic Stem Cells","Humans","Immunoglobulin E","Interleukin-3","Kinetics","Mast Cells","Phospholipases A","Prostaglandin-Endoperoxide Synthases","Recombinant Proteins","Time Factors","Prostaglandin D2","Gene Expression","Leukotriene C4","Stem Cell Factor","Mice","Phospholipases A2"],"keywords":["Prostaglandin D2","Leukotriene C4","Leukotriene","Bone marrow","Immunoglobulin E","Prostaglandin","Population","Chemistry","Molecular biology","Biology","Internal medicine","Endocrinology","Immunology","Antibody","Medicine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-24T12:05:31.787028Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}