{"doi":"10.1073/pnas.95.24.14088","title":"A pyrimidine-rich exonic splicing suppressor binds multiple RNA splicing factors and inhibits spliceosome assembly","abstract":"<jats:p>\n                    The bovine papillomavirus type 1 (BPV-1) exonic splicing suppressor (ESS) is juxtaposed immediately downstream of BPV-1 splicing enhancer 1 and negatively modulates selection of a suboptimal 3′ splice site at nucleotide 3225. The present study demonstrates that this pyrimidine-rich ESS inhibits utilization of upstream 3′ splice sites by blocking early steps in spliceosome assembly. Analysis of the proteins that bind to the ESS showed that the U-rich 5′ region binds U2AF\n                    <jats:sup>65</jats:sup>\n                    and polypyrimidine tract binding protein, the C-rich central part binds 35- and 54–55-kDa serine/arginine-rich (SR) proteins, and the AG-rich 3′ end binds alternative splicing factor/splicing factor 2. Mutational and functional studies indicated that the most critical region of the ESS maps to the central C-rich core (GGCUCCCCC). This core sequence, along with additional nonspecific downstream nucleotides, is sufficient for partial suppression of spliceosome assembly and splicing of BPV-1 pre-mRNAs. The inhibition of splicing by the ESS can be partially relieved by excess purified HeLa SR proteins, suggesting that the ESS suppresses pre-mRNA splicing by interfering with normal bridging and recruitment activities of SR proteins.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1998,"id":606617,"datarank":0.5983476069846413,"base_score":3.9889840465642745,"endowment":3.9889840465642745,"self_citation_contribution":0.5983476069846413,"citation_network_contribution":0.0,"self_endowment_contribution":0.5983476069846413,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":53,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1557352,"name":"Martijn Huynen","orcid":null,"position":1,"is_corresponding":false},{"id":29043,"name":"Carl C. 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Analysis of the proteins that bind to the ESS showed that the U-rich 5′ region binds U2AF\n                    <jats:sup>65</jats:sup>\n                    and polypyrimidine tract binding protein, the C-rich central part binds 35- and 54–55-kDa serine/arginine-rich (SR) proteins, and the AG-rich 3′ end binds alternative splicing factor/splicing factor 2. Mutational and functional studies indicated that the most critical region of the ESS maps to the central C-rich core (GGCUCCCCC). This core sequence, along with additional nonspecific downstream nucleotides, is sufficient for partial suppression of spliceosome assembly and splicing of BPV-1 pre-mRNAs. The inhibition of splicing by the ESS can be partially relieved by excess purified HeLa SR proteins, suggesting that the ESS suppresses pre-mRNA splicing by interfering with normal bridging and recruitment activities of SR proteins.\n                  </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9826658","pmcid":"PMC24331","openalex_id":"https://openalex.org/W9826658","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.00342292,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":null,"oa_locations":[{"url":"https://pnas.org/doi/pdf/10.1073/pnas.95.24.14088","host_type":"publisher"},{"url":"https://dialnet.unirioja.es/servlet/articulo?codigo=2458607","host_type":"journal"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/24331","host_type":"repository"}],"fields_of_study":["Medieval European Literature and History","Religious Tourism and Spaces","Historical, Literary, and Cultural Studies"],"mesh_terms":["Hela Cells","Spliceosomes","Animals","Cattle","Humans","HIV-1","RNA-Binding Proteins","Nuclear Proteins","RNA Precursors","RNA, Viral","Mutagenesis, Site-Directed","Polymerase Chain Reaction","Transcription, Genetic","Alternative Splicing","Sequence Deletion","Base Sequence","Nucleic Acid Conformation","Exons","Templates, Genetic","Molecular Sequence Data","Bovine papillomavirus 1","Enhancer Elements, Genetic","Serine-Arginine Splicing Factors"],"keywords":["History"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T04:52:40.552882Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}