{"doi":"10.1073/pnas.95.11.6267","title":"CIITA stimulation of transcription factor binding to major histocompatibility complex class II and associated promoters\n                    <i>in vivo</i>","abstract":"<jats:p>\n                    CIITA is a master transactivator of the major histocompatibility complex class II genes, which are involved in antigen presentation. Defects in CIITA result in fatal immunodeficiencies. CIITA activation is also the control point for the induction of major histocompatibility complex class II and associated genes by interferon-γ, but CIITA does not bind directly to DNA. Expression of CIITA in G3A cells, which lack endogenous CIITA, followed by\n                    <jats:italic>in vivo</jats:italic>\n                    genomic footprinting, now reveals that CIITA is required for the assembly of transcription factor complexes on the promoters of this gene family, including DRA, Ii, and DMB. CIITA-dependent promoter assembly occurs in interferon-γ-inducible cell types, but not in B lymphocytes. Dissection of the CIITA protein indicates that transactivation and promoter loading are inseparable and reveal a requirement for a GTP binding motif. These findings suggest that CIITA may be a new class of transactivator.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1998,"id":620521,"datarank":0.637274286307404,"base_score":4.248495242049359,"endowment":4.248495242049359,"self_citation_contribution":0.637274286307404,"citation_network_contribution":0.0,"self_endowment_contribution":0.637274286307404,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":69,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":837534,"name":"Keh-Chuang Chin","orcid":null,"position":1,"is_corresponding":false},{"id":1601944,"name":"Michael Linhoff","orcid":null,"position":2,"is_corresponding":false},{"id":837535,"name":"Cheryl Skinner","orcid":null,"position":3,"is_corresponding":false},{"id":1601945,"name":"Jeffrey A. Brown","orcid":null,"position":4,"is_corresponding":false},{"id":270264,"name":"Jeremy M. Boss","orcid":"0000-0002-2432-1840","position":5,"is_corresponding":false},{"id":240329,"name":"George R. Stark","orcid":"0000-0002-8776-9597","position":6,"is_corresponding":false},{"id":531437,"name":"Jenny P.-Y. Ting","orcid":"0000-0003-4398-5164","position":7,"is_corresponding":false},{"id":593302,"name":"Kenneth L. Wright","orcid":"0000-0003-2951-5748","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"CIITA stimulation of transcription factor binding to major histocompatibility complex class II and associated promoters\n                    <i>in vivo</i>","abstract":"<jats:p>\n                    CIITA is a master transactivator of the major histocompatibility complex class II genes, which are involved in antigen presentation. Defects in CIITA result in fatal immunodeficiencies. CIITA activation is also the control point for the induction of major histocompatibility complex class II and associated genes by interferon-γ, but CIITA does not bind directly to DNA. Expression of CIITA in G3A cells, which lack endogenous CIITA, followed by\n                    <jats:italic>in vivo</jats:italic>\n                    genomic footprinting, now reveals that CIITA is required for the assembly of transcription factor complexes on the promoters of this gene family, including DRA, Ii, and DMB. CIITA-dependent promoter assembly occurs in interferon-γ-inducible cell types, but not in B lymphocytes. Dissection of the CIITA protein indicates that transactivation and promoter loading are inseparable and reveal a requirement for a GTP binding motif. These findings suggest that CIITA may be a new class of transactivator.\n                  </jats:p>","is_dataset_classified":null,"base_score":4.248495242049359,"endowment":4.248495242049359,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9600954","pmcid":"PMC27653","openalex_id":"https://openalex.org/W2011451968","authors":[],"funders":[],"total_grants":0,"fwci":4.3727,"citation_percentile":0.95608002,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2016,"count":1},{"year":2019,"count":3},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/27653","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/27653","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.95.11.6267","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.95.11.6267","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/9600954","host_type":"repository"}],"fields_of_study":["Immune Response and Inflammation","NF-κB Signaling Pathways","T-cell and B-cell Immunology","Antigen Presentation","Gene Expression Regulation","Histocompatibility Antigens Class II","Humans","Nuclear Proteins","Protein Binding","Trans-Activators","Transcription Factors","Tumor Cells, Cultured"],"mesh_terms":["Histocompatibility Antigens Class II","Gene Expression Regulation","Humans","Nuclear Proteins","Protein Binding","Transcription Factors","Tumor Cells, Cultured","Trans-Activators","Antigen Presentation"],"keywords":["CIITA","Biology","Transactivation","Promoter","Transcription factor","Major histocompatibility complex","Antigen presentation","Cell biology","Transcription (linguistics)","MHC class II","Gene","Cancer research","Genetics","Gene expression","T cell","Immune system"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T11:19:32.048703Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}