{"doi":"10.1073/pnas.94.8.3837","title":"Gypsy retrotransposon as a tool for the\n            <i>in vivo</i>\n            analysis of the regulatory region of the\n            <i>optomotor-blind</i>\n            gene in \n            <i>Drosophila</i>","abstract":"<jats:p>\n            We report here a method for the\n            <jats:italic>in vivo</jats:italic>\n            dissection of the regulatory region of a gene in the\n            <jats:italic>Drosophila</jats:italic>\n            genome. Our system includes (\n            <jats:italic>i</jats:italic>\n            ) the reporter genes\n            <jats:italic>lacZ</jats:italic>\n            and\n            <jats:italic>white</jats:italic>\n            to detect transcriptional enhancer and silencer activities in a target gene, (\n            <jats:italic>ii</jats:italic>\n            ) an efficient way to induce integration of gypsy elements in the genome, and (\n            <jats:italic>iii</jats:italic>\n            ) unidirectional blocking of regulatory activities by the gypsy element, which is dependent on the\n            <jats:italic>su</jats:italic>\n            (\n            <jats:italic>Hw</jats:italic>\n            ) protein. The\n            <jats:italic>optomotor-blind</jats:italic>\n            (\n            <jats:italic>omb</jats:italic>\n            ) gene was analyzed. In the\n            <jats:italic>omb</jats:italic>\n            <jats:sup>P1</jats:sup>\n            line, a P[\n            <jats:italic>lacW</jats:italic>\n            ] construct is inserted about 1.4 kb upstream of the\n            <jats:italic>omb</jats:italic>\n            transcription start site. The\n            <jats:italic>lacZ</jats:italic>\n            reporter gene within P[\n            <jats:italic>lacW</jats:italic>\n            ] exhibits the same expression pattern as\n            <jats:italic>omb</jats:italic>\n            . The\n            <jats:italic>white</jats:italic>\n            reporter gene is expressed in a “bipolar” pattern. We induced high frequency gypsy mobilization in\n            <jats:italic>omb</jats:italic>\n            <jats:sup>P1</jats:sup>\n            and identified two lines (D11 and D13–1) with altered eye pigmentation pattern, which is dependent on\n            <jats:italic>su</jats:italic>\n            (\n            <jats:italic>Hw</jats:italic>\n            ) activity. A gypsy element was found inserted in the first intron of\n            <jats:italic>omb</jats:italic>\n            in D13–1 and in P[\n            <jats:italic>lacW</jats:italic>\n            ] in D11. These results indicate that it is the blocking of regulatory activities by gypsy that caused the changes in the\n            <jats:italic>white</jats:italic>\n            reporter gene expression. The effect of these gypsy insertions on the expression patterns allowed us to predict several aspects of the organization of the regulatory elements in the\n            <jats:italic>omb</jats:italic>\n            locus.\n          </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1997,"id":25701,"datarank":1.340513908404751,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":0.8911540673716525,"self_endowment_contribution":0.4493598410330987,"citer_contribution":0.8911540673716525,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":13,"citers_with_citation_signal":13,"citers_with_endowment":13,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":150531,"name":"Chuen-Chuen Jang","orcid":null,"position":1,"is_corresponding":false},{"id":150532,"name":"Grigoriy G. Prikhod’ko","orcid":null,"position":2,"is_corresponding":false},{"id":150533,"name":"Dmitri A. Bessarab","orcid":null,"position":3,"is_corresponding":false},{"id":150534,"name":"Chiou-Yang Tang","orcid":null,"position":4,"is_corresponding":false},{"id":150535,"name":"Gert O. Pflugfelder","orcid":null,"position":5,"is_corresponding":false},{"id":150536,"name":"Y. Henry Sun","orcid":null,"position":6,"is_corresponding":false},{"id":150530,"name":"Shih-Feng Tsai","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.995732273553991,"endowment":2.995732273553991,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9108065","pmcid":"PMC20528","openalex_id":"https://openalex.org/W2057334520","authors":[],"funders":[],"total_grants":0,"fwci":1.6042,"citation_percentile":0.85624593,"influential_citations":1,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":2},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2018,"count":2}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/20528","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC20528","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/20528","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.94.8.3837","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.94.8.3837","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/9108065","host_type":"repository"}],"fields_of_study":["Chromosomal and Genetic Variations","CRISPR and Genetic Engineering","Genomics and Chromatin Dynamics","Biology","Medicine","Animals","DNA-Binding Proteins","Drosophila","Drosophila Proteins","Genes, Insect","Genes, Regulator","Nerve Tissue Proteins","Retroelements","T-Box Domain Proteins"],"mesh_terms":["Animals","DNA-Binding Proteins","Drosophila","Genes, Regulator","Nerve Tissue Proteins","Genes, Insect","Retroelements","T-Box Domain Proteins","Drosophila Proteins"],"keywords":["Retrotransposon","Reporter gene","Enhancer","Gene","Biology","Regulatory sequence","Genetics","Gene expression","Regulation of gene expression","Intron","Exon","Molecular biology","Genome","Transposable element"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-08T07:11:29.136524Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}