{"doi":"10.1073/pnas.94.10.4972","title":"Three small nucleolar RNAs that are involved in ribosomal RNA precursor processing","abstract":"<jats:p>\n                    Three small nucleolar RNAs (snoRNAs), E1, E2 and E3, have been described that have unique sequences and interact directly with unique segments of pre-rRNA\n                    <jats:italic>in vivo</jats:italic>\n                    . In this report, injection of antisense oligodeoxynucleotides into\n                    <jats:italic>Xenopus laevis</jats:italic>\n                    oocytes was used to target the specific degradation of these snoRNAs. Specific disruptions of pre-rRNA processing were then observed, which were reversed by injection of the corresponding\n                    <jats:italic>in vitro</jats:italic>\n                    -synthesized snoRNA. Degradation of each of these three snoRNAs produced a unique rRNA maturation phenotype. E1 RNA depletion shut down 18 rRNA formation, without overaccumulation of 20S pre-rRNA. After E2 RNA degradation, production of 18S rRNA and 36S pre-rRNA stopped, and 38S pre-rRNA accumulated, without overaccumulation of 20S pre-rRNA. E3 RNA depletion induced the accumulation of 36S pre-rRNA. This suggests that each of these snoRNAs plays a different role in pre-rRNA processing and indicates that E1 and E2 RNAs are essential for 18S rRNA formation. The available data support the proposal that these snoRNAs are at least involved in pre-rRNA processing at the following pre-rRNA cleavage sites: E1 at the 5′ end and E2 at the 3′ end of 18S rRNA, and E3 at or near the 5′ end of 5.8S rRNA.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1997,"id":632474,"datarank":0.6038027536102726,"base_score":4.02535169073515,"endowment":4.02535169073515,"self_citation_contribution":0.6038027536102726,"citation_network_contribution":0.0,"self_endowment_contribution":0.6038027536102726,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1639472,"name":"George L. Eliceiri","orcid":null,"position":1,"is_corresponding":false},{"id":117659,"name":"Rakesh K. Mishra","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Three small nucleolar RNAs that are involved in ribosomal RNA precursor processing","abstract":"<jats:p>\n                    Three small nucleolar RNAs (snoRNAs), E1, E2 and E3, have been described that have unique sequences and interact directly with unique segments of pre-rRNA\n                    <jats:italic>in vivo</jats:italic>\n                    . In this report, injection of antisense oligodeoxynucleotides into\n                    <jats:italic>Xenopus laevis</jats:italic>\n                    oocytes was used to target the specific degradation of these snoRNAs. Specific disruptions of pre-rRNA processing were then observed, which were reversed by injection of the corresponding\n                    <jats:italic>in vitro</jats:italic>\n                    -synthesized snoRNA. Degradation of each of these three snoRNAs produced a unique rRNA maturation phenotype. E1 RNA depletion shut down 18 rRNA formation, without overaccumulation of 20S pre-rRNA. After E2 RNA degradation, production of 18S rRNA and 36S pre-rRNA stopped, and 38S pre-rRNA accumulated, without overaccumulation of 20S pre-rRNA. E3 RNA depletion induced the accumulation of 36S pre-rRNA. This suggests that each of these snoRNAs plays a different role in pre-rRNA processing and indicates that E1 and E2 RNAs are essential for 18S rRNA formation. The available data support the proposal that these snoRNAs are at least involved in pre-rRNA processing at the following pre-rRNA cleavage sites: E1 at the 5′ end and E2 at the 3′ end of 18S rRNA, and E3 at or near the 5′ end of 5.8S rRNA.\n                  </jats:p>","is_dataset_classified":null,"base_score":4.02535169073515,"endowment":4.02535169073515,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9144174","pmcid":"PMC24615","openalex_id":"https://openalex.org/W2122582252","authors":[],"funders":[],"total_grants":0,"fwci":1.8272,"citation_percentile":0.86126673,"influential_citations":0,"citation_trend":[{"year":2013,"count":2},{"year":2014,"count":1},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":4},{"year":2020,"count":2},{"year":2024,"count":3},{"year":2026,"count":2}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://pnas.org/doi/pdf/10.1073/pnas.94.10.4972","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.94.10.4972","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/9144174","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC24615","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/24615","host_type":"repository"}],"fields_of_study":["RNA modifications and cancer","RNA Research and Splicing","RNA and protein synthesis mechanisms"],"mesh_terms":["Animals","Base Sequence","Cell Nucleolus","Molecular Sequence Data","Nucleic Acid Conformation","Oocytes","Ribosomes","RNA Precursors","RNA Processing, Post-Transcriptional","RNA, Ribosomal","RNA, Small Nuclear","Vertebrates","Xenopus laevis","Oligonucleotides, Antisense","Consensus Sequence"],"keywords":["Small nucleolar RNA","Ribosomal RNA","23S ribosomal RNA","RNA","5.8S ribosomal RNA","18S ribosomal RNA","Biology","Cell biology","Molecular biology","Non-coding RNA","Genetics","Ribosome","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T09:50:09.365876Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}