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We also show that expression of GFP could be monitored in intact living embryos and larvae and in cultured egg chambers, allowing us to visualize dynamic changes in gene expression during real time.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1995,"id":626966,"datarank":0.8504821384470781,"base_score":5.66988092298052,"endowment":5.66988092298052,"self_citation_contribution":0.8504821384470781,"citation_network_contribution":0.0,"self_endowment_contribution":0.8504821384470781,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":289,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1622180,"name":"K Gustafson","orcid":null,"position":1,"is_corresponding":false},{"id":1622181,"name":"G L Boulianne","orcid":null,"position":2,"is_corresponding":false},{"id":569586,"name":"E Yeh","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila.","abstract":"<jats:p>We have used the green fluorescent protein (GFP) from the jellyfish Aequorea victoria as a vital marker/reporter in Drosophila melanogaster. Transgenic flies were generated in which GFP was expressed under the transcriptional control of the yeast upstream activating sequence that is recognized by GAL4. These flies were crossed to several GAL4 enhancer trap lines, and expression of GFP was monitored in a variety of tissues during development using confocal microscopy. Here, we show that GFP could be detected in freshly dissected ovaries, imaginal discs, and the larval nervous system without prior fixation or the addition of substrates or antibodies. We also show that expression of GFP could be monitored in intact living embryos and larvae and in cultured egg chambers, allowing us to visualize dynamic changes in gene expression during real time.</jats:p>","is_dataset_classified":null,"base_score":5.66988092298052,"endowment":5.66988092298052,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7624365","pmcid":"PMC41466","openalex_id":"https://openalex.org/W2080458091","authors":[],"funders":[{"funder_name":"Natural Sciences and Engineering Research Council of Canada","grant_id":"unidentified","title":"unidentified"}],"total_grants":1,"fwci":5.8683,"citation_percentile":0.97610711,"influential_citations":0,"citation_trend":[{"year":2012,"count":12},{"year":2013,"count":9},{"year":2014,"count":5},{"year":2015,"count":6},{"year":2016,"count":8},{"year":2017,"count":10},{"year":2018,"count":6},{"year":2019,"count":13},{"year":2020,"count":3},{"year":2021,"count":3},{"year":2022,"count":9},{"year":2023,"count":4},{"year":2024,"count":2},{"year":2025,"count":1},{"year":2026,"count":2}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://pnas.org/doi/pdf/10.1073/pnas.92.15.7036","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.92.15.7036","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7624365","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC41466","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/41466","host_type":"repository"},{"url":"http://www.pnas.org/content/92/15/7036.full.pdf","host_type":""},{"url":"https://dx.doi.org/10.1073/pnas.92.15.7036","host_type":""}],"fields_of_study":["Neurobiology and Insect Physiology Research","Viral Infectious Diseases and Gene Expression in Insects","Invertebrate Immune Response Mechanisms","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Animals","DNA-Binding Proteins","Drosophila","Enhancer Elements, Genetic","Fungal Proteins","Luminescent Proteins","Oogenesis","Recombinant Fusion Proteins","Tissue Distribution","Transcription Factors","Biomarkers","Gene Expression","Genes, Reporter","Saccharomyces cerevisiae Proteins","Green Fluorescent Proteins"],"keywords":["Aequorea victoria","Green fluorescent protein","Biology","Enhancer trap","Drosophila melanogaster","Reporter gene","Transgene","Enhancer","Cell biology","Gene expression","Marker gene","Molecular biology","Gene","Genetics","Saccharomyces cerevisiae Proteins","Recombinant Fusion Proteins","Green Fluorescent Proteins","DNA-Binding Proteins","Fungal Proteins","Luminescent Proteins","Enhancer Elements, Genetic","Oogenesis","Genes, Reporter","Animals","Drosophila","Tissue Distribution","Biomarkers","Transcription Factors"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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