{"doi":"10.1073/pnas.92.11.5174","title":"A kinase-deficient transcription factor TFIIH is functional in basal and activated transcription.","abstract":"<jats:p>Phosphorylation of the carboxyl-terminal domain (CTD) of the large subunit of RNA polymerase II has been suggested to be critical for transcription initiation, activation, or elongation. A kinase activity specific for CTD is a component of the general transcription factor TFIIH. Recently, a cyclin-dependent kinase-activator kinase (MO15 and cyclin H) was found to be associated with TFIIH preparations and was suggested to be the CTD kinase. TFIIH preparations containing mutant, kinase-deficient MO15 lack CTD kinase activity, indicating that MO15 is critical for polymerase phosphorylation. Nonetheless, these mutant TFIIH preparations were fully functional (in vitro) in both basal and activated transcription. These results indicate that CTD phosphorylation is not required for transcription with a highly purified system.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1995,"id":624126,"datarank":0.7218276533058626,"base_score":4.812184355372417,"endowment":4.812184355372417,"self_citation_contribution":0.7218276533058626,"citation_network_contribution":0.0,"self_endowment_contribution":0.7218276533058626,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":122,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1613259,"name":"J D Parvin","orcid":null,"position":1,"is_corresponding":false},{"id":196666,"name":"J Kim","orcid":null,"position":2,"is_corresponding":false},{"id":1613260,"name":"L J Huber","orcid":null,"position":3,"is_corresponding":false},{"id":117428,"name":"P A Sharp","orcid":null,"position":4,"is_corresponding":false},{"id":1613261,"name":"R A Weinberg","orcid":null,"position":5,"is_corresponding":false},{"id":1613258,"name":"T P Mäkelä","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A kinase-deficient transcription factor TFIIH is functional in basal and activated transcription.","abstract":"<jats:p>Phosphorylation of the carboxyl-terminal domain (CTD) of the large subunit of RNA polymerase II has been suggested to be critical for transcription initiation, activation, or elongation. A kinase activity specific for CTD is a component of the general transcription factor TFIIH. Recently, a cyclin-dependent kinase-activator kinase (MO15 and cyclin H) was found to be associated with TFIIH preparations and was suggested to be the CTD kinase. TFIIH preparations containing mutant, kinase-deficient MO15 lack CTD kinase activity, indicating that MO15 is critical for polymerase phosphorylation. Nonetheless, these mutant TFIIH preparations were fully functional (in vitro) in both basal and activated transcription. These results indicate that CTD phosphorylation is not required for transcription with a highly purified system.</jats:p>","is_dataset_classified":null,"base_score":4.812184355372417,"endowment":4.812184355372417,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7761469","pmcid":"PMC41871","openalex_id":"https://openalex.org/W2043935109","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"P30-CA14051","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P01-CA42063","title":null}],"total_grants":2,"fwci":6.6479,"citation_percentile":0.97596202,"influential_citations":0,"citation_trend":[{"year":2013,"count":2},{"year":2015,"count":2},{"year":2017,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://pnas.org/doi/pdf/10.1073/pnas.92.11.5174","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.92.11.5174","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7761469","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/41871","host_type":"repository"}],"fields_of_study":["Genomics and Chromatin Dynamics","RNA Research and Splicing","RNA modifications and cancer"],"mesh_terms":["Cyclin-Dependent Kinase-Activating Kinase","Bone Neoplasms","Cell Line","DNA Helicases","Humans","Phosphorylation","Protein Kinases","Recombinant Proteins","RNA Polymerase II","Sarcoma","Transcription Factors","Transcription, Genetic","Transfection","Tumor Cells, Cultured","Cyclins","Mutagenesis","Protein Serine-Threonine Kinases","Cyclin-Dependent Kinases","Transcription Factors, TFII","Transcription Factor TFIIH","Cyclin H"],"keywords":["Transcription factor II H","RNA polymerase II","Transcription factor II B","General transcription factor","RNA polymerase II holoenzyme","Cyclin-dependent kinase 9","Transcription factor II A","Biology","Molecular biology","Cyclin-dependent kinase 7","Cell biology","Cyclin-dependent kinase","Cyclin-dependent kinase 2","Phosphorylation","Protein kinase A","Biochemistry","Polymerase","Promoter","RNA-dependent RNA polymerase","Cell cycle","Gene expression","DNA"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T02:46:11.211624Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}