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There is no discernible consensus sequence for the context determinant, suggesting that sequence-specific protein-DNA contacts are not playing a major role. Taken together with previous work, the observations presented suggest a model for the interaction of transposase with the insertion site: symmetrically disposed subunits bind with specific contacts to the major groove of consensus-sequence base pairs, while flanking sequences influence the interaction through effects on DNA helix structure. We also show that the determinants important for insertion into a site are not important for transposition out of that site.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1992,"id":42674,"datarank":5.008355893304764,"base_score":4.454347296253507,"endowment":4.454347296253507,"self_citation_contribution":0.6681520944380261,"citation_network_contribution":4.340203798866738,"self_endowment_contribution":0.6681520944380261,"citer_contribution":4.340203798866738,"corpus_percentile":null,"corpus_rank":null,"citation_count":85,"citer_count":80,"citers_with_citation_signal":72,"citers_with_endowment":72,"datacite_reuse_total":4,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":203944,"name":"N Kleckner","orcid":null,"position":1,"is_corresponding":false},{"id":203943,"name":"J Bender","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.454347296253507,"endowment":4.454347296253507,"datacite_reuse_total":4,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"1325639","pmcid":"PMC49842","openalex_id":"https://openalex.org/W2029664323","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM25326-13","title":null}],"total_grants":1,"fwci":2.1577,"citation_percentile":0.88096515,"influential_citations":5,"citation_trend":[{"year":2012,"count":2},{"year":2014,"count":1},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":3},{"year":2019,"count":2},{"year":2020,"count":3},{"year":2021,"count":1},{"year":2023,"count":1},{"year":2024,"count":3},{"year":2026,"count":2}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://europepmc.org/articles/pmc49842?pdf=render","host_type":"GREEN"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.89.17.7996","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.89.17.7996","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/1325639","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/49842","host_type":"repository"}],"fields_of_study":["RNA and protein synthesis mechanisms","Bacterial Genetics and Biotechnology","Bacteriophages and microbial interactions","Biology","Medicine","Base Sequence","Chromosome Deletion","DNA Mutational Analysis","DNA Transposable Elements","DNA, Bacterial","DNA, Fungal","Histidine","Molecular Sequence Data","Nucleotidyltransferases","Recombination, Genetic","Structure-Activity Relationship","Transposases"],"mesh_terms":["Base Sequence","Chromosome Deletion","DNA Transposable Elements","DNA Mutational Analysis","DNA, Bacterial","DNA, Fungal","Histidine","Molecular Sequence Data","Nucleotidyltransferases","Recombination, Genetic","Structure-Activity Relationship","Transposases"],"keywords":["Tn10","Consensus sequence","Transposable element","Transposase","Insertion sequence","Genetics","Biology","Base pair","Insertion","Transposition (logic)","Sequence (biology)","Context (archaeology)","DNA","Mutation","Mutant","Gene","Base sequence"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.26578181.v1","title":"Additional file 1 of Whole-genome sequencing analysis of two heat-evolved Escherichia coli strains","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.26578181","title":"Additional file 1 of Whole-genome sequencing analysis of two heat-evolved Escherichia coli strains","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.26578184.v1","title":"Additional file 2 of Whole-genome sequencing analysis of two heat-evolved Escherichia coli strains","publisher":"figshare","resource_type":"Dataset"},{"doi":"10.6084/m9.figshare.26578184","title":"Additional file 2 of Whole-genome sequencing analysis of two heat-evolved Escherichia coli strains","publisher":"figshare","resource_type":"Dataset"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-14T04:41:50.564335Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}