{"doi":"10.1073/pnas.89.1.271","title":"Murine B7 antigen provides an efficient costimulatory signal for activation of murine T lymphocytes via the T-cell receptor/CD3 complex.","abstract":"<jats:p>We demonstrate that the murine B7 (mB7) protein is a potent costimulatory molecule for the activation of resting murine CD4+ T cells through the T-cell receptor (TCR)/CD3 complex. Stable mB7-transfected Chinese hamster ovary cells, but not vector-transfected controls, synergize with anti-CD3 monoclonal antibody and Con A-induced T-cell activation, resulting ultimately in proliferation. mB7 exerted its effect by inducing production of interleukin 2 and expression of the interleukin 2 receptor. Thus, mB7 costimulates T-cell activation through the TCR/CD3 complex by positively modulating the normal pathway of T-cell expansion. In contrast to the pronounced effect of mB7 on the activation of T cells through the TCR/CD3 complex, the mB7-transfected CHO cell line costimulated T-cell activation via the glycosylphosphatidylinositol-anchored proteins Thy-1 and Ly-6A.2 only inefficiently. Finally, the combination of a calcium ionophore and mB7 is not sufficient to cause T-cell proliferation, while the combination of a calcium ionophore and phorbol 12-myristate 13-acetate (PMA) stimulates T cells efficiently. The signals that mB7 and PMA provide for murine T lymphocyte activation are therefore not interchangeable, although both costimulate activation through the TCR/CD3 complex.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1992,"id":658240,"datarank":0.7324202883879557,"base_score":4.882801922586371,"endowment":4.882801922586371,"self_citation_contribution":0.7324202883879557,"citation_network_contribution":0.0,"self_endowment_contribution":0.7324202883879557,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":131,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1718307,"name":"G J Freeman","orcid":null,"position":1,"is_corresponding":false},{"id":1718308,"name":"Z Razi-Wolf","orcid":null,"position":2,"is_corresponding":false},{"id":1718309,"name":"C D Gimmi","orcid":null,"position":3,"is_corresponding":false},{"id":1718310,"name":"B Benacerraf","orcid":null,"position":4,"is_corresponding":false},{"id":1718313,"name":"L M Nadler","orcid":null,"position":5,"is_corresponding":false},{"id":1718306,"name":"H Reiser","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Murine B7 antigen provides an efficient costimulatory signal for activation of murine T lymphocytes via the T-cell receptor/CD3 complex.","abstract":"<jats:p>We demonstrate that the murine B7 (mB7) protein is a potent costimulatory molecule for the activation of resting murine CD4+ T cells through the T-cell receptor (TCR)/CD3 complex. Stable mB7-transfected Chinese hamster ovary cells, but not vector-transfected controls, synergize with anti-CD3 monoclonal antibody and Con A-induced T-cell activation, resulting ultimately in proliferation. mB7 exerted its effect by inducing production of interleukin 2 and expression of the interleukin 2 receptor. Thus, mB7 costimulates T-cell activation through the TCR/CD3 complex by positively modulating the normal pathway of T-cell expansion. In contrast to the pronounced effect of mB7 on the activation of T cells through the TCR/CD3 complex, the mB7-transfected CHO cell line costimulated T-cell activation via the glycosylphosphatidylinositol-anchored proteins Thy-1 and Ly-6A.2 only inefficiently. Finally, the combination of a calcium ionophore and mB7 is not sufficient to cause T-cell proliferation, while the combination of a calcium ionophore and phorbol 12-myristate 13-acetate (PMA) stimulates T cells efficiently. The signals that mB7 and PMA provide for murine T lymphocyte activation are therefore not interchangeable, although both costimulate activation through the TCR/CD3 complex.</jats:p>","is_dataset_classified":null,"base_score":4.882801922586371,"endowment":4.882801922586371,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"1370349","pmcid":"PMC48218","openalex_id":"https://openalex.org/W2057167188","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA-46967","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI-30169","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA-40216","title":null}],"total_grants":3,"fwci":14.6814,"citation_percentile":0.99328636,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2018,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/48218","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/48218","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.89.1.271","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.89.1.271","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/1370349","host_type":"repository"}],"fields_of_study":["Monoclonal and Polyclonal Antibodies Research","T-cell and B-cell Immunology","Immunotherapy and Immune Responses"],"mesh_terms":["Calcimycin","Animals","Antigens, Differentiation, T-Lymphocyte","Antigens, Ly","Antigens, Surface","Glycolipids","Cricetinae","Interleukin-2","Lymphocyte Activation","Phosphatidylinositols","Receptors, Antigen, T-Cell","Tetradecanoylphorbol Acetate","Transfection","Blotting, Northern","Receptors, Interleukin-2","Signal Transduction","CD4-Positive T-Lymphocytes","Up-Regulation","CHO Cells","CD3 Complex","Glycosylphosphatidylinositols","B7-1 Antigen","Thy-1 Antigens","Mice"],"keywords":["Biology","Chinese hamster ovary cell","Interleukin 2","T cell","T-cell receptor","Molecular biology","T lymphocyte","CD3","Transfection","Antigen","Jurkat cells","Cell biology","Cell culture","Receptor","CD8","Immune system","Biochemistry","Immunology"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T03:40:32.495617Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}