{"doi":"10.1073/pnas.88.12.5072","title":"A chimeric mammalian transactivator based on the lac repressor that is regulated by temperature and isopropyl beta-D-thiogalactopyranoside.","abstract":"<jats:p>LAP267 is a lacI activator protein (LAP) containing an insertion of the transcriptional activation domain of the herpes simplex virus virion protein 16 within the inducer-binding and dimerization domain of the lac repressor protein. LAP267 strongly induces expression in a conditional manner from a minimal simian virus 40 early promoter linked to lac operator sequences. LAP267 is temperature-sensitive, activating expression at 32 degrees C but not at 39.5 degrees C. It is allosterically regulated in a manner opposite that of wild-type lac repressor, in that LAP267 activity is rescued at the nonpermissive temperature by isopropyl beta-D-thiogalactopyranoside (IPTG). Stable mouse cell lines containing both the LAP267 gene and a LAP-inducible chloramphenicol acetyltransferase (CAT) reporter gene were readily established and exhibited up to a 1200-fold increase in CAT activity within 24 hr upon addition of IPTG. Thus, LAP267 is a powerful inducible switch in mammalian cells, imparting a regulatory stringency similar to that observed with lac repressor in Escherichia coli.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1991,"id":31897,"datarank":7.909610674121446,"base_score":4.59511985013459,"endowment":4.59511985013459,"self_citation_contribution":0.6892679775201885,"citation_network_contribution":7.220342696601257,"self_endowment_contribution":0.6892679775201885,"citer_contribution":7.220342696601257,"corpus_percentile":null,"corpus_rank":null,"citation_count":98,"citer_count":96,"citers_with_citation_signal":87,"citers_with_endowment":87,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":169960,"name":"M A Labow","orcid":null,"position":1,"is_corresponding":false},{"id":169962,"name":"A J Levine","orcid":null,"position":2,"is_corresponding":false},{"id":169964,"name":"T Shenk","orcid":null,"position":3,"is_corresponding":false},{"id":169958,"name":"S B Baim","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.59511985013459,"endowment":4.59511985013459,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"2052587","pmcid":"PMC51813","openalex_id":"https://openalex.org/W2058303636","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA41086","title":null}],"total_grants":1,"fwci":2.0687,"citation_percentile":0.88106587,"influential_citations":7,"citation_trend":[{"year":2012,"count":1},{"year":2014,"count":1},{"year":2015,"count":1},{"year":2016,"count":3},{"year":2019,"count":1},{"year":2020,"count":5},{"year":2021,"count":1},{"year":2023,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://europepmc.org/articles/pmc51813?pdf=render","host_type":"GREEN"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.88.12.5072","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.88.12.5072","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/2052587","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/51813","host_type":"repository"}],"fields_of_study":["Toxin Mechanisms and Immunotoxins","Herpesvirus Infections and Treatments","Transgenic Plants and Applications","Medicine","Biology","Chemistry","Animals","Chimera","Chloramphenicol O-Acetyltransferase","DNA","Electrophoresis, Polyacrylamide Gel","Enzyme Induction","HeLa Cells","Humans","Isopropyl Thiogalactoside","Kinetics","Mammals","Repressor Proteins","Temperature","Trans-Activators","Transfection"],"mesh_terms":["Animals","Chimera","DNA","Electrophoresis, Polyacrylamide Gel","Enzyme Induction","HeLa Cells","Humans","Isopropyl Thiogalactoside","Kinetics","Mammals","Repressor Proteins","Temperature","Transfection","Chloramphenicol O-Acetyltransferase","Trans-Activators","Hela Cells"],"keywords":["Lac repressor","lac operon","Repressor","Chloramphenicol acetyltransferase","Molecular biology","Activator (genetics)","Reporter gene","Gene","Chemistry","Transactivation","Inducer","Escherichia coli","Biology","Beta-galactosidase","Gene expression","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T09:07:45.293740Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}