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These findings indicate that the reconstituted replication reaction has many of the characteristics expected of authentic viral DNA replication.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1990,"id":619418,"datarank":0.7612760722850741,"base_score":5.075173815233827,"endowment":5.075173815233827,"self_citation_contribution":0.7612760722850741,"citation_network_contribution":0.0,"self_endowment_contribution":0.7612760722850741,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":159,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1598463,"name":"K L Collins","orcid":null,"position":1,"is_corresponding":false},{"id":1598464,"name":"P Simancek","orcid":null,"position":2,"is_corresponding":false},{"id":823834,"name":"A Russo","orcid":null,"position":3,"is_corresponding":false},{"id":1598465,"name":"M S Wold","orcid":null,"position":4,"is_corresponding":false},{"id":1598466,"name":"D M Virshup","orcid":null,"position":5,"is_corresponding":false},{"id":1598467,"name":"T J Kelly","orcid":null,"position":6,"is_corresponding":false},{"id":1598462,"name":"D H Weinberg","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Reconstitution of simian virus 40 DNA replication with purified proteins.","abstract":"<jats:p>Replication of plasmid DNA molecules containing the simian virus 40 (SV40) origin of DNA replication has been reconstituted with seven highly purified cellular proteins plus the SV40 large tumor (T) antigen. Initiation of DNA synthesis is absolutely dependent upon T antigen, replication protein A, and the DNA polymerase alpha-primase complex and is stimulated by the catalytic subunit of protein phosphatase 2A. Efficient elongation of nascent chains additionally requires proliferating cell nuclear antigen, replication factor C, DNA topoisomerase I, and DNA polymerase delta. Electron microscopic studies indicate that DNA replication begins at the viral origin and proceeds via intermediates containing two forks that move in opposite directions. These findings indicate that the reconstituted replication reaction has many of the characteristics expected of authentic viral DNA replication.</jats:p>","is_dataset_classified":null,"base_score":5.075173815233827,"endowment":5.075173815233827,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"2174160","pmcid":"PMC55025","openalex_id":"https://openalex.org/W1988939915","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA 16519","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA 40414","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"DK-01528","title":null}],"total_grants":3,"fwci":18.4047,"citation_percentile":0.99346861,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":1},{"year":2014,"count":1},{"year":2015,"count":2},{"year":2016,"count":2},{"year":2017,"count":1},{"year":2019,"count":1},{"year":2021,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://pnas.org/doi/pdf/10.1073/pnas.87.22.8692","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.87.22.8692","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/2174160","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/55025","host_type":"repository"}],"fields_of_study":["Polyomavirus and related diseases","Bacteriophages and microbial interactions","DNA Repair Mechanisms","Antigens, Polyomavirus Transforming","Cell-Free System","DNA Replication","In Vitro Techniques","Microscopy, Electron","Proteins","RNA, Viral","Simian virus 40"],"mesh_terms":["Antigens, Polyomavirus Transforming","Cell-Free System","DNA Replication","Microscopy, Electron","Proteins","RNA, Viral","Simian virus 40","In Vitro Techniques"],"keywords":["Replication factor C","Control of chromosome duplication","Eukaryotic DNA replication","Origin recognition complex","DNA replication","DNA polymerase II","DNA polymerase","DNA polymerase delta","Biology","Primase","DNA clamp","Molecular biology","Replication protein A","DNA replication factor CDT1","Pre-replication complex","DNA","Ter protein","Proliferating cell nuclear antigen","Genetics","DNA-binding protein","Reverse transcriptase","Polymerase chain reaction","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T07:06:01.584176Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}