{"doi":"10.1073/pnas.83.21.8044","title":"Location of regulatory elements responsible for drug induction in the rat cytochrome P-450c gene.","abstract":"<jats:p>The synthesis of cytochrome P-450c is induced remarkably in cultured cells as well as animal tissues in response to added chemicals such as 3-methylcholanthrene and 2,3,7,8-tetrachlorodibenzodioxin. To study this mechanism, we joined the sequence of 5'-flanking and upstream regions of the P-450c gene to the structural gene for chloramphenicol acetyltransferase. The fusion gene was introduced into Hepa-1 cells for the assay of the expressed acetyltransferase activity. At least three cis-acting regulatory regions that are responsible for the inductive expression were determined in the sequences from nucleotide -3674 to -3067, from -1682 to -1429, and from -1139 to -1029, relative to the transcription start site, by external deletion analysis. Further detailed analysis of the region (nucleotides -1139 to -1029) most influential on the inducibility revealed that a regulatory element consisting of 10 base pairs termed a drug regulatory element (DRE) and its homologues were tandemly arranged in this region. The consensus sequence deduced from DREs is 5'-GCNTGAGGCTGGG-3'. The regulatory sequence from nucleotide -1140 to -844 is capable of conferring inducibility on a heterologous promoter in a manner independent of its orientation and distance from the subordinate promoter.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1986,"id":20787,"datarank":8.344605510021,"base_score":4.948759890378168,"endowment":4.948759890378168,"self_citation_contribution":0.7423139835567254,"citation_network_contribution":7.602291526464274,"self_endowment_contribution":0.7423139835567254,"citer_contribution":7.602291526464274,"corpus_percentile":null,"corpus_rank":null,"citation_count":140,"citer_count":132,"citers_with_citation_signal":131,"citers_with_endowment":131,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":136238,"name":"A Fujisawa-Sehara","orcid":null,"position":1,"is_corresponding":false},{"id":136239,"name":"M Yamane","orcid":null,"position":2,"is_corresponding":false},{"id":136240,"name":"Y Fujii-Kuriyama","orcid":null,"position":3,"is_corresponding":false},{"id":136237,"name":"K Sogawa","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.948759890378168,"endowment":4.948759890378168,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"3464941","pmcid":"PMC386863","openalex_id":"https://openalex.org/W2083848894","authors":[],"funders":[],"total_grants":0,"fwci":13.0789,"citation_percentile":0.98986682,"influential_citations":4,"citation_trend":[{"year":2021,"count":3},{"year":2022,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/386863","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc386863?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/386863","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.83.21.8044","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.83.21.8044","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/3464941","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC386863","host_type":"repository"}],"fields_of_study":["Pharmacogenetics and Drug Metabolism","Chemical Reactions and Isotopes","Carcinogens and Genotoxicity Assessment","Biology","Medicine","Acetyltransferases","Animals","Base Sequence","Chloramphenicol O-Acetyltransferase","Cytochrome P-450 Enzyme System","Enzyme Induction","Gene Expression Regulation","Genes, Regulator","Methylcholanthrene","Mutation","Promoter Regions, Genetic","Rats"],"mesh_terms":["Acetyltransferases","Animals","Base Sequence","Cytochrome P-450 Enzyme System","Enzyme Induction","Gene Expression Regulation","Genes, Regulator","Methylcholanthrene","Mutation","Promoter Regions, Genetic","Chloramphenicol O-Acetyltransferase","Rats"],"keywords":["Chloramphenicol acetyltransferase","Regulatory sequence","Gene","Biology","Genetics","Nucleic acid sequence","Molecular biology","Nucleotide","Promoter","Consensus sequence","Transcription factor","Gene expression","Peptide sequence"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-06T11:17:25.043518Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}