{"doi":"10.1073/pnas.81.20.6325","title":"Molecular cloning and characterization of DNA sequences encoding rat and human atrial natriuretic factors.","abstract":"<jats:p>A cDNA copy of the message encoding rat atrial natriuretic factor (ANF) has been cloned in Escherichia coli, and its nucleotide sequence was determined. ANF appears to be synthesized as a larger precursor, atrial pronatriodilatin. The cDNA has an open reading frame potentially encoding a protein of 152 amino acids, of which the first 24 amino acids strongly resemble a signal sequence. This is followed by a sequence with 80% homology to a second vasoactive protein, porcine cardiodilatin. The ANF peptide is contained in the COOH-terminal portion of the protein. The DNA sequence corresponding to human ANF is also presented and displays a high degree of homology to its rat counterpart. These data provide further evidence for the expression in cardiac atria of a multifactor system that may contribute to the regulation of blood pressure and extracellular fluid volume.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1984,"id":34667,"datarank":7.859984907482318,"base_score":4.844187086458591,"endowment":4.844187086458591,"self_citation_contribution":0.7266280629687888,"citation_network_contribution":7.1333568445135285,"self_endowment_contribution":0.7266280629687888,"citer_contribution":7.1333568445135285,"corpus_percentile":null,"corpus_rank":null,"citation_count":126,"citer_count":109,"citers_with_citation_signal":101,"citers_with_endowment":101,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":179192,"name":"J H Condra","orcid":null,"position":1,"is_corresponding":false},{"id":179193,"name":"R A Dixon","orcid":null,"position":2,"is_corresponding":false},{"id":179194,"name":"N G Seidah","orcid":null,"position":3,"is_corresponding":false},{"id":179195,"name":"M Chrétien","orcid":null,"position":4,"is_corresponding":false},{"id":179196,"name":"M Nemer","orcid":null,"position":5,"is_corresponding":false},{"id":179197,"name":"M Chamberland","orcid":null,"position":6,"is_corresponding":false},{"id":179198,"name":"J Drouin","orcid":null,"position":7,"is_corresponding":false},{"id":179191,"name":"R A Zivin","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.844187086458591,"endowment":4.844187086458591,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"6238331","pmcid":"PMC391916","openalex_id":"https://openalex.org/W2052248858","authors":[],"funders":[],"total_grants":0,"fwci":8.0674,"citation_percentile":0.98342449,"influential_citations":3,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/391916","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc391916?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/391916","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.81.20.6325","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.81.20.6325","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/6238331","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC391916","host_type":"repository"}],"fields_of_study":["RNA and protein synthesis mechanisms","Cardiomyopathy and Myosin Studies","CRISPR and Genetic Engineering","Biology","Medicine","Amino Acid Sequence","Animals","Atrial Natriuretic Factor","Base Sequence","Cloning, Molecular","DNA","Genes","Humans","Muscle Proteins","Natriuresis","Natriuretic Agents","Protein Precursors","Proteins","RNA, Messenger","Rats"],"mesh_terms":["Amino Acid Sequence","Animals","Base Sequence","Cloning, Molecular","DNA","Genes","Humans","Muscle Proteins","Natriuresis","Atrial Natriuretic Factor","Protein Precursors","Proteins","RNA, Messenger","Natriuretic Agents","Rats"],"keywords":["Complementary DNA","Molecular cloning","Peptide sequence","Open reading frame","Biology","Nucleic acid sequence","Signal peptide","Homology (biology)","Amino acid","Molecular biology","DNA","NPR2","Escherichia coli","Cloning (programming)","Biochemistry","Gene","Natriuretic peptide","Internal medicine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T19:30:59.679217Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}