{"doi":"10.1073/pnas.79.10.3167","title":"Mechanism of action of dichloro-beta-D-ribofuranosylbenzimidazole: effect on in vitro transcription.","abstract":"<jats:p>The adenosine analog 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) and its mono- and triphosphate derivatives inhibit RNA polymerase II-specific transcription in an extract of whole HeLa cells. The analog does not inhibit RNA polymerase III-specific adenovirus VA RNA transcription in the whole cell extract. With purified RNA polymerase II under nonspecific transcription conditions, no effect on DRB could be detected. DRB is equally effective in inhibiting in vitro transcription from several of the adenovirus promoters and the human epsilon-globin gene. The inhibitory effects are in the order DRB greater than DRB monophosphate greater than DRB triphosphate. Thus DRB acts in vitro presumably on systems in which specific RNA polymerase II initiation of transcription occurs and with no detectable effect on premature termination. This will provide a suitable model for study of the molecular mechanism of action of DRB on transcription.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1982,"id":45718,"datarank":6.39825523072562,"base_score":4.553876891600541,"endowment":4.553876891600541,"self_citation_contribution":0.6830815337400812,"citation_network_contribution":5.715173696985539,"self_endowment_contribution":0.6830815337400812,"citer_contribution":5.715173696985539,"corpus_percentile":null,"corpus_rank":null,"citation_count":94,"citer_count":87,"citers_with_citation_signal":85,"citers_with_endowment":85,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":213159,"name":"B Mittleman","orcid":null,"position":1,"is_corresponding":false},{"id":213160,"name":"D Bunick","orcid":null,"position":2,"is_corresponding":false},{"id":213161,"name":"S Ackerman","orcid":null,"position":3,"is_corresponding":false},{"id":213162,"name":"R Weinmann","orcid":null,"position":4,"is_corresponding":false},{"id":213158,"name":"R Zandomeni","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.553876891600541,"endowment":4.553876891600541,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"6954467","pmcid":"PMC346375","openalex_id":"https://openalex.org/W2048463752","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA-21124","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA-10815","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI-13231","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01AI013231-09","title":"CONTROL OF ADENOVIRUS TRANSCRIPTION"},{"funder_name":"National Institutes of Health","grant_id":"2T32CA009171-21","title":"TRAINING PROGRAM IN BASIC CANCER RESEARCH"}],"total_grants":5,"fwci":2.2296,"citation_percentile":0.88146202,"influential_citations":4,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1},{"year":2015,"count":2},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2023,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/346375","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc346375?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/346375","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.79.10.3167","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.79.10.3167","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/6954467","host_type":"repository"},{"url":"http://www.pnas.org/content/79/10/3167.full.pdf","host_type":""},{"url":"https://dx.doi.org/10.1073/pnas.79.10.3167","host_type":""}],"fields_of_study":["RNA modifications and cancer","RNA and protein synthesis mechanisms","RNA regulation and disease","Biology","Medicine","Chemistry","0301 basic medicine","0303 health sciences","03 medical and health sciences","Cell-Free System","DNA-Directed RNA Polymerases","Dichlororibofuranosylbenzimidazole","HeLa Cells","Humans","Phosphorylation","RNA Polymerase II","Ribonucleosides","Transcription, Genetic"],"mesh_terms":["Cell-Free System","Dichlororibofuranosylbenzimidazole","HeLa Cells","Humans","Phosphorylation","Ribonucleosides","RNA Polymerase II","DNA-Directed RNA Polymerases","Transcription, Genetic","Hela Cells"],"keywords":["RNA polymerase II","Transcription (linguistics)","Molecular biology","RNA polymerase","Transcription factor II D","Transcription preinitiation complex","Biology","General transcription factor","RNA polymerase II holoenzyme","Polymerase","RNA polymerase III","Transcription factor II B","RNA","Chemistry","Gene expression","Promoter","Biochemistry","Gene","Cell-Free System","Transcription, Genetic","Humans","DNA-Directed RNA Polymerases","Ribonucleosides","Phosphorylation","Dichlororibofuranosylbenzimidazole","HeLa Cells"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-04T00:28:11.473703Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}