{"doi":"10.1073/pnas.77.12.7157","title":"Receptor structure in the bacterial sensing system.","abstract":"<jats:p>The primary receptors for aspartate and serine in bacterial chemotaxis have been shown to be the 60,000-dalton proteins encoded by the tar and tsr genes. The evidence is: (i) overproduction of the tar gene product at various levels by recombinant DNA techniques produces proportionate increases in aspartate binding; (ii) aspartate binding copurifies with [3H]methyl-labeled tar gene product; (iii) antibody to tar and tsr protein fragments precipitates a single species of protein (60,000 daltons) which retains binding capacity and [3H]carboxymethyl label. Partially purified tar gene product can be reconstituted into artificial vesicles and retains aspartate binding and aspartate-sensitive methylation and demethylation. These results show that the aspartate and serine receptors are transmembrane proteins of a single polypeptide chain with the receptor recognition site on the outside of the membrane and the covalent methylation site on the inside.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1980,"id":611375,"datarank":0.7117398192544876,"base_score":4.74493212836325,"endowment":4.74493212836325,"self_citation_contribution":0.7117398192544876,"citation_network_contribution":0.0,"self_endowment_contribution":0.7117398192544876,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":114,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1573415,"name":"D E Koshland","orcid":null,"position":1,"is_corresponding":false},{"id":1573414,"name":"E A Wang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Receptor structure in the bacterial sensing system.","abstract":"<jats:p>The primary receptors for aspartate and serine in bacterial chemotaxis have been shown to be the 60,000-dalton proteins encoded by the tar and tsr genes. The evidence is: (i) overproduction of the tar gene product at various levels by recombinant DNA techniques produces proportionate increases in aspartate binding; (ii) aspartate binding copurifies with [3H]methyl-labeled tar gene product; (iii) antibody to tar and tsr protein fragments precipitates a single species of protein (60,000 daltons) which retains binding capacity and [3H]carboxymethyl label. Partially purified tar gene product can be reconstituted into artificial vesicles and retains aspartate binding and aspartate-sensitive methylation and demethylation. These results show that the aspartate and serine receptors are transmembrane proteins of a single polypeptide chain with the receptor recognition site on the outside of the membrane and the covalent methylation site on the inside.</jats:p>","is_dataset_classified":null,"base_score":4.74493212836325,"endowment":4.74493212836325,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"6784119","pmcid":"PMC350460","openalex_id":"https://openalex.org/W1999462600","authors":[],"funders":[{"funder_name":"NIADDK NIH HHS","grant_id":"AM-9765","title":null}],"total_grants":1,"fwci":10.1107,"citation_percentile":0.99202586,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/350460","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/350460","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.77.12.7157","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.77.12.7157","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/6784119","host_type":"repository"}],"fields_of_study":["Advanced Proteomics Techniques and Applications","Bacterial Genetics and Biotechnology","RNA and protein synthesis mechanisms","Aspartic Acid","Bacterial Proteins","Chemotaxis","DNA, Recombinant","Escherichia coli","Genes","Macromolecular Substances","Protein Binding","Receptors, Drug","Serine"],"mesh_terms":["Aspartic Acid","Bacterial Proteins","Chemotaxis","DNA, Recombinant","Escherichia coli","Genes","Protein Binding","Receptors, Drug","Serine","Macromolecular Substances"],"keywords":["Biochemistry","Gene product","Receptor","Serine","Methylation","Biology","Membrane protein","Chemistry","Gene","Molecular biology","Gene expression","Membrane","Phosphorylation"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-01T18:55:53.846305Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}