{"doi":"10.1073/pnas.76.12.6309","title":"Attractants and repellents influence methylation and demethylation of methyl-accepting chemotaxis proteins in an extract of Escherichia coli.","abstract":"<jats:p>During bacterial chemotaxis, attractants and repellents alter the methylation levels of the methyl-accepting chemotaxis proteins (MCPs). These methylation levels represent a balance between two enzymatic processes: methylation and demethylation. In vivo experiments previously have shown that chemoeffectors influence the demethylation process; effects on the methylation system have not been reported. Here we show that in a cell-free extract of Escherichia coli both methylation and demethylation of the MCPs are affected by attractants and repellents. Attractants enhance methylation and inhibit demethylation. Repellents inhibit methylation and stimulate demethylation. The cell-free system provides an opportunity for further study of the mechanisms by which attractants and repellents influence the levels of methylation of the MCPs.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":1979,"id":588653,"datarank":3.7637584660986763,"base_score":3.912023005428146,"endowment":3.912023005428146,"self_citation_contribution":0.586803450814222,"citation_network_contribution":3.176955015284454,"self_endowment_contribution":0.586803450814222,"citer_contribution":3.176955015284454,"corpus_percentile":null,"corpus_rank":null,"citation_count":49,"citer_count":47,"citers_with_citation_signal":43,"citers_with_endowment":43,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1505985,"name":"A C Hobson","orcid":null,"position":1,"is_corresponding":false},{"id":1269035,"name":"J Adler","orcid":null,"position":2,"is_corresponding":false},{"id":1505984,"name":"S J Kleene","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Attractants and repellents influence methylation and demethylation of methyl-accepting chemotaxis proteins in an extract of Escherichia coli.","abstract":"<jats:p>During bacterial chemotaxis, attractants and repellents alter the methylation levels of the methyl-accepting chemotaxis proteins (MCPs). These methylation levels represent a balance between two enzymatic processes: methylation and demethylation. In vivo experiments previously have shown that chemoeffectors influence the demethylation process; effects on the methylation system have not been reported. Here we show that in a cell-free extract of Escherichia coli both methylation and demethylation of the MCPs are affected by attractants and repellents. Attractants enhance methylation and inhibit demethylation. Repellents inhibit methylation and stimulate demethylation. The cell-free system provides an opportunity for further study of the mechanisms by which attractants and repellents influence the levels of methylation of the MCPs.</jats:p>","is_dataset_classified":null,"base_score":3.912023005428146,"endowment":3.912023005428146,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"392517","pmcid":"PMC411853","openalex_id":"https://openalex.org/W2048794814","authors":[],"funders":[],"total_grants":0,"fwci":1.1596,"citation_percentile":0.76440894,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2014,"count":3},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":3},{"year":2022,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/411853","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/411853","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.76.12.6309","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.76.12.6309","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/392517","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC411853","host_type":"repository"}],"fields_of_study":["Protein Structure and Dynamics","Epigenetics and DNA Methylation","Cancer-related gene regulation","Bacterial Proteins","Cell-Free System","Chemoreceptor Cells","Chemotaxis","Escherichia coli","Kinetics","Methylation","S-Adenosylmethionine"],"mesh_terms":["Bacterial Proteins","Cell-Free System","Chemoreceptor Cells","Chemotaxis","Escherichia coli","Kinetics","Methylation","S-Adenosylmethionine"],"keywords":["Demethylation","Methylation","Escherichia coli","Chemotaxis","Protein methylation","Biology","Biochemistry","DNA methylation","Chemistry","Methyltransferase","Gene expression","Gene","Receptor"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-22T16:21:27.678923Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}