{"doi":"10.1073/pnas.252646399","title":"CUL7: A DOC domain-containing cullin selectively binds Skp1⋅Fbx29 to form an SCF-like complex","abstract":"<jats:p>Selective protein degradation targeted by members of the F-box protein family plays pivotal roles in cell biology. It is widely accepted that an F-box protein directs substrate ubiquitination within a Skp1⋅CUL1⋅F-box protein⋅ROC1 (SCF-ROC1) E3 ubiquitin ligase complex. This assembly utilizes the CUL1 molecular scaffold, allowing the F-box protein to position its bound substrate for ubiquitination by a ROC1-recruited E2-conjugating enzyme. Here, we describe an alternative mechanism for assembling an F-box protein-based E3 complex through a previously uncharacterized cullin, CUL7, identified by mass spectrometry as a ROC1-interacting protein. CUL7 is a large polypeptide containing a cullin domain, which is responsible for ROC1 binding, and a DOC domain, which is also present in the anaphase-promoting complex. Remarkably, CUL7 assembles an SCF-ROC1-like E3 ubiquitin ligase complex consisting of Skp1, CUL7, the Fbx29 F-box protein, and ROC1. In contrast to CUL1 that binds Skp1 by itself, CUL7 interacts with the Skp1⋅Fbx29 complex, but not with Skp1 alone. Strikingly, CUL7 selectively interacts with Skp1⋅Fbx29 but not with Skp1⋅βTRCP2 or Skp1⋅Skp2. Thus, CUL7 may define a previously uncharacterized, Fbx29-mediated, and ubiquitin-dependent proteolysis pathway.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2002,"id":24046,"datarank":8.882135249088677,"base_score":5.147494476813453,"endowment":5.147494476813453,"self_citation_contribution":0.7721241715220181,"citation_network_contribution":8.110011077566659,"self_endowment_contribution":0.7721241715220181,"citer_contribution":8.110011077566659,"corpus_percentile":null,"corpus_rank":null,"citation_count":171,"citer_count":147,"citers_with_citation_signal":132,"citers_with_endowment":132,"datacite_reuse_total":14,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":145518,"name":"Georgia Dolios","orcid":null,"position":1,"is_corresponding":false},{"id":145519,"name":"Rong Wang","orcid":null,"position":2,"is_corresponding":false},{"id":145520,"name":"Zhen-Qiang Pan","orcid":null,"position":3,"is_corresponding":false},{"id":145517,"name":"Dora C. 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and proteasome pathways","Autophagy in Disease and Therapy","Endoplasmic Reticulum Stress and Disease","Biology","Medicine","Binding Sites","Cell Cycle Proteins","Cullin Proteins","Humans","Ligases","Peptide Synthases","S-Phase Kinase-Associated Proteins","SKP Cullin F-Box Protein Ligases","Ubiquitin-Protein Ligases"],"mesh_terms":["Binding Sites","Humans","Ligases","Peptide Synthases","Cell Cycle Proteins","Ubiquitin-Protein Ligases","S-Phase Kinase-Associated Proteins","Cullin Proteins","SKP Cullin F-Box Protein Ligases"],"keywords":["Skp1","Cullin","Ubiquitin ligase","F-box protein","Cell division control protein 4","Ubiquitin","Biology","Cell biology","Chemistry","Biochemistry","Molecular biology"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.26564865.v1","title":"Additional file 1 of 14-3-3 proteins regulate cullin 7-mediated Eag1 degradation","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.26564865","title":"Additional file 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