{"doi":"10.1073/pnas.2437756100","title":"Regulation of voltage-gated calcium channel activity by the Rem and Rad GTPases","abstract":"<jats:p>\n                    Rem, Rem2, Rad, and Gem/Kir (RGK) represent a distinct GTPase family with largely unknown physiological functions. We report here that both Rem and Rad bind directly to Ca\n                    <jats:sup>2+</jats:sup>\n                    channel β-subunits (Ca\n                    <jats:sub>V</jats:sub>\n                    β)\n                    <jats:italic>in vivo</jats:italic>\n                    . No calcium currents are recorded from human embryonic kidney 293 cells coexpressing the L type Ca\n                    <jats:sup>2+</jats:sup>\n                    channel subunits Ca\n                    <jats:sub>V</jats:sub>\n                    1.2, Ca\n                    <jats:sub>V</jats:sub>\n                    β\n                    <jats:sub>2a</jats:sub>\n                    , and Rem or Rad, but Ca\n                    <jats:sub>V</jats:sub>\n                    1.2 and Ca\n                    <jats:sub>V</jats:sub>\n                    β\n                    <jats:sub>2a</jats:sub>\n                    transfected cells elicit Ca\n                    <jats:sup>2+</jats:sup>\n                    channel currents in the absence of these small G proteins. Importantly, Ca\n                    <jats:sub>V</jats:sub>\n                    3 (T type) Ca\n                    <jats:sup>2+</jats:sup>\n                    channels, which do not require accessory subunits for ionic current expression, are not inhibited by expression of Rem. Rem is expressed in primary skeletal myoblasts and, when overexpressed in C2C12 myoblasts, wild-type Rem inhibits L type Ca\n                    <jats:sup>2+</jats:sup>\n                    channel activity. Deletion analysis demonstrates a critical role for the Rem C terminus in both regulation of functional Ca\n                    <jats:sup>2+</jats:sup>\n                    channel expression and β-subunit association. These results suggest that all members of the RGK GTPase family, via direct interaction with auxiliary β-subunits, serve as regulators of L type Ca\n                    <jats:sup>2+</jats:sup>\n                    channel activity. Thus, the RGK GTPase family may provide a mechanism for achieving cross talk between Ras-related GTPases and electrical signaling pathways.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2003,"id":39900,"datarank":9.36627140504804,"base_score":5.3612921657094255,"endowment":5.3612921657094255,"self_citation_contribution":0.8041938248564139,"citation_network_contribution":8.562077580191627,"self_endowment_contribution":0.8041938248564139,"citer_contribution":8.562077580191627,"corpus_percentile":null,"corpus_rank":null,"citation_count":212,"citer_count":186,"citers_with_citation_signal":163,"citers_with_endowment":163,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":194892,"name":"Shawn M. 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