{"doi":"10.1073/pnas.2416722121","title":"AMBRA1 controls the translation of immune-specific genes in T lymphocytes","abstract":"T cell receptor (TCR) engagement causes a global cellular response that entrains signaling pathways, cell cycle regulation, and cell death. The molecular regulation of mRNA translation in these processes is poorly understood. Using a whole-genome CRISPR screen for regulators of CD95 (FAS/APO-1)-mediated T cell death, we identified AMBRA1, a protein previously studied for its roles in autophagy, E3 ubiquitin ligase activity, and cyclin regulation. T cells lacking AMBRA1 resisted FAS-mediated cell death by down-regulating FAS expression at the translational level. We show that AMBRA1 is a vital regulator of ribosome protein biosynthesis and ribosome loading on select mRNAs, whereby it plays a key role in balancing TCR signaling with cell cycle regulation pathways. We also found that AMBRA1 itself is translationally controlled by TCR stimulation via the CD28-PI3K-mTORC1-EIF4F pathway. Together, these findings shed light on the molecular control of translation after T cell activation and implicate AMBRA1 as a translational regulator governing TCR signaling, cell cycle progression, and T cell death.","journal":"Proceedings of the National Academy of Sciences","year":2024,"id":445801,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9519,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1204602,"name":"Wanjing Shang","orcid":"0009-0007-0646-7946","position":1,"is_corresponding":false},{"id":1262185,"name":"Deji Ye","orcid":"0000-0002-5161-8359","position":2,"is_corresponding":false},{"id":720354,"name":"Satoshi Kubo","orcid":"0000-0001-9693-9263","position":3,"is_corresponding":false},{"id":138847,"name":"Ping Jiang","orcid":null,"position":4,"is_corresponding":false},{"id":748447,"name":"Samantha Shafer","orcid":"0000-0001-9429-414X","position":5,"is_corresponding":false},{"id":1034702,"name":"Leilei Xu","orcid":"0000-0002-2467-8612","position":6,"is_corresponding":false},{"id":1262186,"name":"Lixin Zheng","orcid":"0000-0003-0760-4176","position":7,"is_corresponding":false},{"id":720357,"name":"Ann Y. Park","orcid":"0000-0002-0130-1521","position":8,"is_corresponding":false},{"id":1052274,"name":"Jian Song","orcid":"0000-0002-0289-3063","position":9,"is_corresponding":false},{"id":573676,"name":"Waipan Chan","orcid":"0000-0002-9109-6583","position":10,"is_corresponding":false},{"id":1262623,"name":"Zhiqin Zeng","orcid":null,"position":11,"is_corresponding":false},{"id":298051,"name":"Tingyan He","orcid":"0000-0002-0222-2091","position":12,"is_corresponding":false},{"id":108651,"name":"Benjamin Schwarz","orcid":"0000-0002-5894-953X","position":13,"is_corresponding":false},{"id":323800,"name":"Björn Häupl","orcid":"0009-0000-9445-9565","position":14,"is_corresponding":false},{"id":32727,"name":"Thomas Oellerich","orcid":"0000-0001-6570-6125","position":15,"is_corresponding":false},{"id":103764,"name":"Michael J. Lenardo","orcid":"0000-0003-1584-468X","position":16,"is_corresponding":false},{"id":573678,"name":"Yikun Yao","orcid":"0000-0002-8890-2409","position":17,"is_corresponding":false},{"id":1262184,"name":"Simone Gottlieb","orcid":"0000-0003-0550-4761","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:01:46.243344Z","pmid":"39436665","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}