{"doi":"10.1073/pnas.2416712122","title":"In vivo Pirt-Marina voltage sensor imaging detects primary sensory neuron–specific voltage dynamics and neuronal plasticity changes","abstract":"In vivo voltage imaging is a powerful tool for monitoring action potentials and dynamic electrical events in heterogeneous sensory neurons enabling the deciphering of rapid somatosensory information processing. Virus-driven expression of genetically encoded voltage indicator (GEVI) suffers from inconsistent expression levels and offers a limited time window for optimal voltage imaging. Here, we generated and characterized a knock-in mouse line with Pirt-driven expression of Marina, a positively tuned GEVI, in primary sensory neurons. Pirt-Marina mice enable optical reporting of touch, itch, and nociceptive sensations in vivo and distinct action potential patterns in the trigeminal and dorsal root ganglion neurons. Notably, Pirt-Marina mice display robust fluorescence signals in response to mechanical, thermal, or chemical stimuli, allowing visualization of transformations in sensory coding following inflammation and injury. This Pirt-Marina mouse line provides optical access to dynamic neuronal activity and plasticity in the peripheral nervous system (PNS) with high temporal accuracy, fidelity, and reliability.","journal":"Proceedings of the National Academy of Sciences","year":2025,"id":546856,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.952,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":695785,"name":"Hyeonwi Son","orcid":null,"position":1,"is_corresponding":false},{"id":694919,"name":"John Shannonhouse","orcid":"0000-0002-2749-1140","position":2,"is_corresponding":false},{"id":832235,"name":"Rubén Sánchez Gómez","orcid":"0000-0002-9326-0332","position":3,"is_corresponding":false},{"id":1439347,"name":"Eungyung Kim","orcid":null,"position":4,"is_corresponding":false},{"id":1439348,"name":"Chih-Hsuan Ai","orcid":null,"position":5,"is_corresponding":false},{"id":246682,"name":"Man‐Kyo Chung","orcid":"0000-0001-7637-1148","position":6,"is_corresponding":false},{"id":567009,"name":"Jelena Platiša","orcid":"0000-0003-4813-609X","position":7,"is_corresponding":false},{"id":567011,"name":"Vincent A. Pieribone","orcid":"0000-0002-5836-5454","position":8,"is_corresponding":false},{"id":694920,"name":"Yu Shin Kim","orcid":"0000-0002-6677-5656","position":9,"is_corresponding":false},{"id":694918,"name":"Yan Zhang","orcid":"0000-0002-4159-2044","position":0,"is_corresponding":true}],"reference_count":43,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:53:36.567932Z","pmid":"40938705","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}