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Inactivating the HUSH–SETDB1–MORC2 complex before infection significantly reduces H3K9me3 on the viral genome, with minimal impact on the cellular genome, aside from expected changes in LINE-1 retroelements. Depletion of HUSH, SETDB1, or MORC2 alleviates HSV-1 repression in infected primary human fibroblasts and human induced pluripotent stem cell–derived sensory neurons (hiPSDN). We found that the viral protein ICP0 induces MORC2 degradation via the proteasome machinery. This process is concurrent with ICP0 and MORC2 depletion capability to reactivate silenced HSV-1 in hiPSDN. Overall, our findings underscore the robust antiviral function of the HUSH–SETDB1–MORC2 repressor complex against a herpesvirus by modulating chromatin marks linked to repression, thus presenting promising avenues for anti-herpesvirus therapeutic strategies.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2024,"id":662448,"datarank":0.40620753016533157,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.0,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1729406,"name":"Tristan Escure","orcid":"0000-0002-0599-5727","position":1,"is_corresponding":false},{"id":728242,"name":"Franceline Juillard","orcid":"0000-0003-1449-3603","position":2,"is_corresponding":false},{"id":1604218,"name":"Armelle Corpet","orcid":"0000-0002-2126-5783","position":3,"is_corresponding":false},{"id":1729407,"name":"Rémi Néplaz","orcid":"0009-0004-3488-1720","position":4,"is_corresponding":false},{"id":125449,"name":"Olivier Binda","orcid":"0000-0002-1539-0828","position":5,"is_corresponding":false},{"id":1729408,"name":"Coline Seurre","orcid":null,"position":6,"is_corresponding":false},{"id":1729409,"name":"Mathilde Gonin","orcid":"0009-0004-3507-1148","position":7,"is_corresponding":false},{"id":643366,"name":"Stuart Bloor","orcid":"0009-0000-3194-3042","position":8,"is_corresponding":false},{"id":288277,"name":"Camille Cohen","orcid":"0000-0001-6939-8393","position":9,"is_corresponding":false},{"id":1729411,"name":"Pascale Texier","orcid":null,"position":10,"is_corresponding":false},{"id":1729412,"name":"Oscar Haigh","orcid":null,"position":11,"is_corresponding":false},{"id":1729413,"name":"Olivier Pascual","orcid":null,"position":12,"is_corresponding":false},{"id":1729414,"name":"Yonatan Ganor","orcid":null,"position":13,"is_corresponding":false},{"id":490797,"name":"Frédérique Magdinier","orcid":"0000-0002-0159-9559","position":14,"is_corresponding":false},{"id":1729415,"name":"Marc Labetoulle","orcid":null,"position":15,"is_corresponding":false},{"id":614690,"name":"Paul J. 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Using a cellular model that replicates vDCP NB formation, we previously demonstrated that these viral genomes are chromatinized with the H3.3 histone variant modified on its lysine 9 by trimethylation (H3.3K9me3), a mark associated with transcriptional repression. Here, we identify the HUSH complex and its effectors, SETDB1 and MORC2, as crucial for the acquisition of H3K9me3 on PML NB-associated HSV-1 and the maintenance of HSV-1 transcriptional repression. ChIP-seq analyses show H3K9me3 association with the entire viral genome. Inactivating the HUSH–SETDB1–MORC2 complex before infection significantly reduces H3K9me3 on the viral genome, with minimal impact on the cellular genome, aside from expected changes in LINE-1 retroelements. Depletion of HUSH, SETDB1, or MORC2 alleviates HSV-1 repression in infected primary human fibroblasts and human induced pluripotent stem cell–derived sensory neurons (hiPSDN). We found that the viral protein ICP0 induces MORC2 degradation via the proteasome machinery. This process is concurrent with ICP0 and MORC2 depletion capability to reactivate silenced HSV-1 in hiPSDN. 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