{"doi":"10.1073/pnas.2319057121","title":"CCR3-dependent eosinophil recruitment is regulated by sialyltransferase ST3Gal-IV","abstract":"Eosinophil recruitment is a pathological hallmark of many allergic and helminthic diseases. Here, we investigated chemokine receptor CCR3-induced eosinophil recruitment in sialyltransferase St3gal4 −/− mice. We found a marked decrease in eosinophil extravasation into CCL11-stimulated cremaster muscles and into the inflamed peritoneal cavity of St3gal4 −/− mice. Ex vivo flow chamber assays uncovered reduced adhesion of St3gal4 −/− compared to wild type eosinophils. Using flow cytometry, we show reduced binding of CCL11 to St3gal4 −/− eosinophils. Further, we noted reduced binding of CCL11 to its chemokine receptor CCR3 isolated from St3gal4 −/− eosinophils. This was accompanied by almost absent CCR3 internalization of CCL11-stimulated St3gal4 −/− eosinophils. Applying an ovalbumin-induced allergic airway disease model, we found a dramatic reduction in eosinophil numbers in bronchoalveolar lavage fluid following intratracheal challenge with ovalbumin in St3gal4 -deficient mice. Finally, we also investigated tissue-resident eosinophils under homeostatic conditions and found reduced resident eosinophil numbers in the thymus and adipose tissue in the absence of ST3Gal-IV. Taken together, our results demonstrate an important role of ST3Gal-IV in CCR3-induced eosinophil recruitment in vivo rendering this enzyme an attractive target in reducing unwanted eosinophil infiltration in various disorders including allergic diseases.","journal":"Proceedings of the National Academy of Sciences","year":2024,"id":435193,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9464,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1242839,"name":"Katrin Nussbaumer","orcid":null,"position":1,"is_corresponding":false},{"id":1242840,"name":"Axel Doerner","orcid":null,"position":2,"is_corresponding":false},{"id":319640,"name":"Omar El Bounkari","orcid":"0000-0002-3051-2096","position":3,"is_corresponding":false},{"id":1242363,"name":"Silke Huber","orcid":"0000-0002-7021-1845","position":4,"is_corresponding":false},{"id":1242841,"name":"Janine Abisch","orcid":null,"position":5,"is_corresponding":false},{"id":1242364,"name":"Matteo Napoli","orcid":"0000-0002-8197-9374","position":6,"is_corresponding":false},{"id":504888,"name":"Sarah Schmidt","orcid":"0000-0002-4200-9703","position":7,"is_corresponding":false},{"id":859237,"name":"Andreas Margraf","orcid":"0000-0002-6539-6478","position":8,"is_corresponding":false},{"id":666322,"name":"Monika Pruenster","orcid":"0000-0001-8573-6866","position":9,"is_corresponding":false},{"id":667184,"name":"Ina Rohwedder","orcid":null,"position":10,"is_corresponding":false},{"id":1242365,"name":"Bärbel Lange-Sperandio","orcid":"0000-0001-8898-3459","position":11,"is_corresponding":false},{"id":277383,"name":"Marcus Mall","orcid":"0000-0002-4057-2199","position":12,"is_corresponding":false},{"id":1214249,"name":"Renske de Jong","orcid":null,"position":13,"is_corresponding":false},{"id":1242366,"name":"Caspar Ohnmacht","orcid":"0000-0002-6887-8819","position":14,"is_corresponding":false},{"id":1242842,"name":"Juergen Bernhagen","orcid":null,"position":15,"is_corresponding":false},{"id":527350,"name":"David Voehringer","orcid":"0000-0001-6650-0639","position":16,"is_corresponding":false},{"id":266074,"name":"Jamey D. Marth","orcid":"0000-0001-9323-1649","position":17,"is_corresponding":false},{"id":1242367,"name":"David Frommhold","orcid":"0000-0002-7289-1346","position":18,"is_corresponding":false},{"id":392167,"name":"Markus Sperandio","orcid":"0000-0002-7689-3613","position":19,"is_corresponding":false},{"id":666315,"name":"Roland Immler","orcid":"0000-0002-4086-7528","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":null,"created_at":"2026-07-19T02:00:03.406367Z","pmid":"38687790","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}