{"doi":"10.1073/pnas.2315659121","title":"Transcription factor C/EBPα is required for the development of Ly6C <sup>hi</sup> monocytes but not Ly6C <sup>lo</sup> monocytes","abstract":"Monocytes comprise two major subsets, Ly6C hi classical monocytes and Ly6C lo nonclassical monocytes. Notch2 signaling in Ly6C hi monocytes triggers transition to Ly6C lo monocytes, which require Nr4a1 , Bcl6 , Irf2 , and Cebpb . By comparison, less is known about transcriptional requirements for Ly6C hi monocytes. We find transcription factor CCAAT/enhancer-binding protein alpha (C/EBPα) is highly expressed in Ly6C hi monocytes, but down-regulated in Ly6C lo monocytes. A few previous studies described the requirement of C/EBPα in the development of neutrophils and eosinophils. However, the role of C/EBPα for in vivo monocyte development has not been understood. We deleted the Cebpa +37 kb enhancer in mice, eliminating hematopoietic expression of C/EBPα, reproducing the expected neutrophil defect. Surprisingly, we also found a severe and selective loss of Ly6C hi monocytes, while preserving Ly6C lo monocytes. We find that BM progenitors from Cebpa +37 −/− mice rapidly progress through the monocyte progenitor stage to develop directly into Ly6C lo monocytes even in the absence of Notch2 signaling. These results identify a previously unrecognized role for C/EBPα in maintaining Ly6C hi monocyte identity.","journal":"Proceedings of the National Academy of Sciences","year":2024,"id":423262,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9588,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":699736,"name":"Jing Chen","orcid":"0000-0002-5998-6930","position":1,"is_corresponding":false},{"id":395806,"name":"Feiya Ou","orcid":"0000-0001-8289-4025","position":2,"is_corresponding":false},{"id":299070,"name":"Tiantian Liu","orcid":"0000-0001-9377-9938","position":3,"is_corresponding":false},{"id":1043116,"name":"Suin Jo","orcid":"0000-0002-7575-9017","position":4,"is_corresponding":false},{"id":235670,"name":"William E. Gillanders","orcid":"0000-0002-0465-3071","position":5,"is_corresponding":false},{"id":299074,"name":"Theresa L. Murphy","orcid":"0000-0001-6539-8142","position":6,"is_corresponding":false},{"id":227901,"name":"Kenneth M. Murphy","orcid":"0000-0002-3505-9630","position":7,"is_corresponding":false},{"id":299067,"name":"Sun Kyung Kim","orcid":"0000-0002-1375-6339","position":0,"is_corresponding":true}],"reference_count":75,"raw_metadata":null,"created_at":"2026-07-19T01:57:56.814994Z","pmid":"38564635","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}