{"doi":"10.1073/pnas.2314245121","title":"Elf1 promotes Rad26’s interaction with lesion-arrested Pol II for transcription-coupled repair","abstract":"Transcription-coupled nucleotide excision repair (TC-NER) is a highly conserved DNA repair pathway that removes bulky lesions in the transcribed genome. Cockayne syndrome B protein (CSB), or its yeast ortholog Rad26, has been known for decades to play important roles in the lesion-recognition steps of TC-NER. Another conserved protein ELOF1, or its yeast ortholog Elf1, was recently identified as a core transcription-coupled repair factor. How Rad26 distinguishes between RNA polymerase II (Pol II) stalled at a DNA lesion or other obstacles and what role Elf1 plays in this process remains unknown. Here, we present cryo-EM structures of Pol II-Rad26 complexes stalled at different obstacles that show that Rad26 uses a common mechanism to recognize a stalled Pol II, with additional interactions when Pol II is arrested at a lesion. A cryo-EM structure of lesion-arrested Pol II-Rad26 bound to Elf1 revealed that Elf1 induces further interactions between Rad26 and a lesion-arrested Pol II. Biochemical and genetic data support the importance of the interplay between Elf1 and Rad26 in TC-NER initiation. Together, our results provide important mechanistic insights into how two conserved transcription-coupled repair factors, Rad26/CSB and Elf1/ELOF1, work together at the initial lesion recognition steps of transcription-coupled repair.","journal":"Proceedings of the National Academy of Sciences","year":2024,"id":429928,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9533,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":498748,"name":"Jun Xu","orcid":"0009-0001-1360-2778","position":1,"is_corresponding":false},{"id":239982,"name":"Indrajit Lahiri","orcid":"0000-0002-5633-7978","position":2,"is_corresponding":false},{"id":1137261,"name":"Wenzhi Gong","orcid":"0009-0007-8518-2593","position":3,"is_corresponding":false},{"id":817892,"name":"Qingrong Li","orcid":"0000-0002-8583-3620","position":4,"is_corresponding":false},{"id":498747,"name":"Juntaek Oh","orcid":"0000-0002-7625-2779","position":5,"is_corresponding":false},{"id":823336,"name":"Zhen Zhou","orcid":"0000-0003-0706-9089","position":6,"is_corresponding":false},{"id":1232588,"name":"Peini Hou","orcid":"0000-0002-5214-8029","position":7,"is_corresponding":false},{"id":498749,"name":"Jenny Chong","orcid":"0000-0002-4182-188X","position":8,"is_corresponding":false},{"id":494015,"name":"Nan Hao","orcid":"0000-0003-2857-4789","position":9,"is_corresponding":false},{"id":1137262,"name":"Shisheng Li","orcid":"0000-0002-2380-2132","position":10,"is_corresponding":false},{"id":498750,"name":"Dong Wang","orcid":"0000-0002-2829-1546","position":11,"is_corresponding":false},{"id":239990,"name":"Andrés E. Leschziner","orcid":"0000-0002-7732-7023","position":12,"is_corresponding":false},{"id":1232587,"name":"Reta D. Sarsam","orcid":"0000-0003-3816-2470","position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":null,"created_at":"2026-07-19T01:59:11.328802Z","pmid":"38194460","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}