{"doi":"10.1073/pnas.2221114120","title":"Robust regulation of transcription pausing in  <i>Escherichia coli</i> by the ubiquitous elongation factor NusG","abstract":"Transcription elongation by multi-subunit RNA polymerases (RNAPs) is regulated by auxiliary factors in all organisms. NusG/Spt5 is the only universally conserved transcription elongation factor shared by all domains of life. NusG is a component of antitermination complexes controlling ribosomal RNA operons, an essential antipausing factor, and a transcription-translation coupling factor in Escherichia coli . We employed RNET-seq for genome-wide mapping of RNAP pause sites in wild-type and NusG-depleted cells. We demonstrate that NusG is a major antipausing factor that suppresses thousands of backtracked and nonbacktracked pauses across the E. coli genome. The NusG-suppressed pauses were enriched immediately downstream from the translation start codon but were also abundant elsewhere in open reading frames, small RNA genes, and antisense transcription units. This finding revealed a strong similarity of NusG to Spt5, which stimulates the elongation rate of many eukaryotic genes. We propose a model in which promoting forward translocation and/or stabilization of RNAP in the posttranslocation register by NusG results in suppression of pausing in E. coli .","journal":"Proceedings of the National Academy of Sciences","year":2023,"id":337321,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9584,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1068668,"name":"Mikhail Bubunenko","orcid":"0009-0004-7906-9488","position":1,"is_corresponding":false},{"id":576497,"name":"Zachary F. Mandell","orcid":null,"position":2,"is_corresponding":false},{"id":1069165,"name":"Lucyna Lubkowska","orcid":null,"position":3,"is_corresponding":false},{"id":1069166,"name":"Sara Husher","orcid":null,"position":4,"is_corresponding":false},{"id":392645,"name":"Paul Babitzke","orcid":"0000-0003-2481-1062","position":5,"is_corresponding":false},{"id":392644,"name":"Mikhail Kashlev","orcid":"0000-0002-1260-6486","position":6,"is_corresponding":false},{"id":392643,"name":"Alexander V. Yakhnin","orcid":"0000-0002-7313-7054","position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":null,"created_at":"2026-07-19T01:10:26.690308Z","pmid":"37276387","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}