{"doi":"10.1073/pnas.2216006120","title":"Intrinsically disordered proteins SAID1/2 condensate on SERRATE for dual inhibition of miRNA biogenesis in Arabidopsis","abstract":"Intrinsically disordered proteins (IDPs) SAID1/2 are hypothetic dentin sialophosphoprotein-like proteins, but their true functions are unknown. Here, we identified SAID1/2 as negative regulators of SERRATE (SE), a core factor in miRNA biogenesis complex (microprocessor). Loss-of-function double mutants of said1; said2 caused pleiotropic developmental defects and thousands of differentially expressed genes that partially overlapped with those in se. said1; said2 also displayed increased assembly of microprocessor and elevated accumulation of microRNAs (miRNAs). Mechanistically, SAID1/2 promote pre-mRNA processing 4 kinase A-mediated phosphorylation of SE, causing its degradation in vivo. Unexpectedly, SAID1/2 have strong binding affinity to hairpin-structured pri-miRNAs and can sequester them from SE. Moreover, SAID1/2 directly inhibit pri-miRNA processing by microprocessor in vitro. Whereas SAID1/2 did not impact SE subcellular compartmentation, the proteins themselves exhibited liquid–liquid phase condensation that is nucleated on SE. Thus, we propose that SAID1/2 reduce miRNA production through hijacking pri-miRNAs to prevent microprocessor activity while promoting SE phosphorylation and its destabilization in Arabidopsis.","journal":"Proceedings of the National Academy of Sciences","year":2023,"id":339492,"datarank":0.42498200160843247,"base_score":2.833213344056216,"endowment":2.833213344056216,"self_citation_contribution":0.42498200160843247,"citation_network_contribution":0.0,"self_endowment_contribution":0.42498200160843247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9615,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":476449,"name":"Lin Wang","orcid":"0000-0001-7787-904X","position":1,"is_corresponding":false},{"id":722020,"name":"Xingxing Yan","orcid":"0000-0002-0140-4029","position":2,"is_corresponding":false},{"id":722019,"name":"Yanjun Li","orcid":"0000-0003-3822-6470","position":3,"is_corresponding":false},{"id":720567,"name":"Changhao Li","orcid":"0009-0006-1581-3866","position":4,"is_corresponding":false},{"id":1073460,"name":"Chaohua Wu","orcid":null,"position":5,"is_corresponding":false},{"id":1073071,"name":"Tian Wang","orcid":"0000-0002-2147-2459","position":6,"is_corresponding":false},{"id":499102,"name":"Xiang Guo","orcid":"0009-0005-9940-9037","position":7,"is_corresponding":false},{"id":1073072,"name":"Sukwon Choi","orcid":"0000-0001-9372-1947","position":8,"is_corresponding":false},{"id":1073461,"name":"Tianru Zhang","orcid":null,"position":9,"is_corresponding":false},{"id":1073073,"name":"Ziying Wang","orcid":"0009-0008-1378-543X","position":10,"is_corresponding":false},{"id":651402,"name":"Chun-Yip Tong","orcid":"0000-0001-8886-6463","position":11,"is_corresponding":false},{"id":1073074,"name":"Taerin Oh","orcid":"0009-0000-4549-8322","position":12,"is_corresponding":false},{"id":690542,"name":"Xiao Zhang","orcid":"0000-0002-8599-3181","position":13,"is_corresponding":false},{"id":1073075,"name":"Zhiye Wang","orcid":"0000-0002-2328-3117","position":14,"is_corresponding":false},{"id":720568,"name":"Xu Peng","orcid":"0000-0001-9068-102X","position":15,"is_corresponding":false},{"id":537733,"name":"Xiuren Zhang","orcid":"0000-0001-8982-2999","position":16,"is_corresponding":false},{"id":722022,"name":"Baoshuan Shang","orcid":"0000-0002-8744-3554","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:10:45.048445Z","pmid":"36972460","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}