{"doi":"10.1073/pnas.2202815120","title":"The capsid lattice engages a bipartite NUP153 motif to mediate nuclear entry of HIV-1 cores","abstract":"Increasing evidence has suggested that the HIV-1 capsid enters the nucleus in a largely assembled, intact form. However, not much is known about how the cone-shaped capsid interacts with the nucleoporins (NUPs) in the nuclear pore for crossing the nuclear pore complex. Here, we elucidate how NUP153 binds HIV-1 capsid by engaging the assembled capsid protein (CA) lattice. A bipartite motif containing both canonical and noncanonical interaction modules was identified at the C-terminal tail region of NUP153. The canonical cargo-targeting phenylalanine-glycine (FG) motif engaged the CA hexamer. By contrast, a previously unidentified triple-arginine (RRR) motif in NUP153 targeted HIV-1 capsid at the CA tri-hexamer interface in the capsid. HIV-1 infection studies indicated that both FG- and RRR-motifs were important for the nuclear import of HIV-1 cores. Moreover, the presence of NUP153 stabilized tubular CA assemblies in vitro. Our results provide molecular-level mechanistic evidence that NUP153 contributes to the entry of the intact capsid into the nucleus.","journal":"Proceedings of the National Academy of Sciences","year":2023,"id":320967,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":46,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9586,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1032379,"name":"Sushila Kumari","orcid":"0000-0003-0827-7517","position":1,"is_corresponding":false},{"id":307285,"name":"Chaoyi Xu","orcid":"0000-0002-9870-4297","position":2,"is_corresponding":false},{"id":561574,"name":"Sooin Jang","orcid":"0000-0003-2937-0948","position":3,"is_corresponding":false},{"id":710386,"name":"Jiong Shi","orcid":"0000-0002-8684-4268","position":4,"is_corresponding":false},{"id":1032380,"name":"Ryan C. Burdick","orcid":"0000-0002-0290-5896","position":5,"is_corresponding":false},{"id":732590,"name":"Lev Levintov","orcid":"0000-0002-7950-9226","position":6,"is_corresponding":false},{"id":561738,"name":"Qiancheng Xiong","orcid":"0000-0002-9897-7391","position":7,"is_corresponding":false},{"id":621847,"name":"Chunxiang Wu","orcid":"0000-0002-8635-1578","position":8,"is_corresponding":false},{"id":246107,"name":"Swapnil C. Devarkar","orcid":"0000-0002-9271-243X","position":9,"is_corresponding":false},{"id":561739,"name":"Taoran Tian","orcid":"0000-0002-8048-0328","position":10,"is_corresponding":false},{"id":461459,"name":"Therese N. Tripler","orcid":"0000-0001-9336-7271","position":11,"is_corresponding":false},{"id":246106,"name":"Yingxia Hu","orcid":"0000-0003-1395-0016","position":12,"is_corresponding":false},{"id":246103,"name":"Shuai Yuan","orcid":"0000-0003-4037-3429","position":13,"is_corresponding":false},{"id":461458,"name":"Joshua Temple","orcid":"0000-0002-1351-5690","position":14,"is_corresponding":false},{"id":433162,"name":"Qingzhou Feng","orcid":null,"position":15,"is_corresponding":false},{"id":249705,"name":"C. Patrick Lusk","orcid":"0000-0003-4703-0533","position":16,"is_corresponding":false},{"id":307290,"name":"Christopher Aiken","orcid":"0000-0002-2476-4078","position":17,"is_corresponding":false},{"id":228712,"name":"Alan Engelman","orcid":"0000-0002-9709-2591","position":18,"is_corresponding":false},{"id":298286,"name":"Juan R. Perilla","orcid":"0000-0003-1171-6816","position":19,"is_corresponding":false},{"id":409359,"name":"Vinay K. Pathak","orcid":"0000-0003-2441-8412","position":20,"is_corresponding":false},{"id":556299,"name":"Chenxiang Lin","orcid":"0000-0001-7041-1946","position":21,"is_corresponding":false},{"id":246111,"name":"Yong Xiong","orcid":"0000-0001-9625-9313","position":22,"is_corresponding":false},{"id":244531,"name":"Qi Shen","orcid":"0000-0002-9062-8955","position":0,"is_corresponding":true}],"reference_count":83,"raw_metadata":null,"created_at":"2026-07-19T01:07:27.434133Z","pmid":"36943880","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}