{"doi":"10.1073/pnas.212633199","title":"Dicer is required for chromosome segregation and gene silencing in fission yeast cells","abstract":"<jats:p>\n                    RNA interference is a form of gene silencing in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs. Here we report a role for Dicer in chromosome segregation of fission yeast. Deletion of the Dicer (\n                    <jats:italic>dcr1</jats:italic>\n                    <jats:sup>+</jats:sup>\n                    ) gene caused slow growth, sensitivity to thiabendazole, lagging chromosomes during anaphase, and abrogated silencing of centromeric repeats. As Dicer in other species, Dcr1p degraded double-stranded RNA into ≈23 nucleotide fragments\n                    <jats:italic>in vitro</jats:italic>\n                    , and\n                    <jats:italic>dcr1</jats:italic>\n                    Δ cells were partially rescued by expression of human Dicer, indicating evolutionarily conserved functions. Expression profiling demonstrated that\n                    <jats:italic>dcr1</jats:italic>\n                    <jats:sup>+</jats:sup>\n                    was required for silencing of two genes containing a conserved motif.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2002,"id":651731,"datarank":0.7412463633913957,"base_score":4.941642422609304,"endowment":4.941642422609304,"self_citation_contribution":0.7412463633913957,"citation_network_contribution":0.0,"self_endowment_contribution":0.7412463633913957,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":139,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1699860,"name":"Rebecca A. 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Here we report a role for Dicer in chromosome segregation of fission yeast. Deletion of the Dicer (\n                    <jats:italic>dcr1</jats:italic>\n                    <jats:sup>+</jats:sup>\n                    ) gene caused slow growth, sensitivity to thiabendazole, lagging chromosomes during anaphase, and abrogated silencing of centromeric repeats. As Dicer in other species, Dcr1p degraded double-stranded RNA into ≈23 nucleotide fragments\n                    <jats:italic>in vitro</jats:italic>\n                    , and\n                    <jats:italic>dcr1</jats:italic>\n                    Δ cells were partially rescued by expression of human Dicer, indicating evolutionarily conserved functions. Expression profiling demonstrated that\n                    <jats:italic>dcr1</jats:italic>\n                    <jats:sup>+</jats:sup>\n                    was required for silencing of two genes containing a conserved motif.\n                  </jats:p>","is_dataset_classified":null,"base_score":4.941642422609304,"endowment":4.941642422609304,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"12482946","pmcid":"PMC139198","openalex_id":"https://openalex.org/W2023845765","authors":[],"funders":[],"total_grants":0,"fwci":20.0773,"citation_percentile":0.99271718,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":1},{"year":2014,"count":4},{"year":2015,"count":1},{"year":2016,"count":2},{"year":2017,"count":5},{"year":2018,"count":3},{"year":2020,"count":4},{"year":2021,"count":6},{"year":2022,"count":4},{"year":2023,"count":2},{"year":2024,"count":3},{"year":2025,"count":1},{"year":2026,"count":2}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://www.pnas.org/doi/pdf/10.1073/pnas.212633199","host_type":"journal"},{"url":"https://www.pnas.org/doi/pdf/10.1073/pnas.212633199","host_type":"publisher"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.212633199","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.212633199","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/12482946","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/139198","host_type":"repository"}],"fields_of_study":["Plant Virus Research Studies","Plant Disease Resistance and Genetics","RNA Research and Splicing","Chromosome Segregation","Endoribonucleases","Gene Silencing","Humans","Reverse Transcriptase Polymerase Chain Reaction","Ribonuclease III","Schizosaccharomyces"],"mesh_terms":["Endoribonucleases","Humans","Schizosaccharomyces","Chromosome Segregation","Reverse Transcriptase Polymerase Chain Reaction","Gene Silencing","Ribonuclease III"],"keywords":["Dicer","Biology","Gene silencing","RNA-induced silencing complex","RNA-induced transcriptional silencing","RNA silencing","RNA interference","Ribonuclease III","Molecular biology","Argonaute","Genetics","Small interfering RNA","Cell biology","Gene","RNA"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T10:32:21.330254Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}