{"doi":"10.1073/pnas.2122364119","title":"Photosynthetic biohybrid coculture for tandem and tunable CO <sub>2</sub> and N <sub>2</sub> fixation","abstract":"Solar-driven bioelectrosynthesis represents a promising approach for converting abundant resources into value-added chemicals with renewable energy. Microorganisms powered by electrochemical reducing equivalents assimilate CO 2 , H 2 O, and N 2 building blocks. However, products from autotrophic whole-cell biocatalysts are limited. Furthermore, biocatalysts tasked with N 2 reduction are constrained by simultaneous energy-intensive autotrophy. To overcome these challenges, we designed a biohybrid coculture for tandem and tunable CO 2 and N 2 fixation to value-added products, allowing the different species to distribute bioconversion steps and reduce the individual metabolic burden. This consortium involves acetogen Sporomusa ovata , which reduces CO 2 to acetate, and diazotrophic Rhodopseudomonas palustris , which uses the acetate both to fuel N 2 fixation and for the generation of a biopolyester. We demonstrate that the coculture platform provides a robust ecosystem for continuous CO 2 and N 2 fixation, and its outputs are directed by substrate gas composition. Moreover, we show the ability to support the coculture on a high–surface area silicon nanowire cathodic platform. The biohybrid coculture achieved peak faradaic efficiencies of 100, 19.1, and 6.3% for acetate, nitrogen in biomass, and ammonia, respectively, while maintaining product tunability. Finally, we established full solar to chemical conversion driven by a photovoltaic device, resulting in solar to chemical efficiencies of 1.78, 0.51, and 0.08% for acetate, nitrogenous biomass, and ammonia, correspondingly. Ultimately, our work demonstrates the ability to employ and electrochemically manipulate bacterial communities on demand to expand the suite of CO 2 and N 2 bioelectrosynthesis products.","journal":"Proceedings of the National Academy of Sciences","year":2022,"id":237658,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":59,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9493,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":860446,"name":"Rachel R. Chan","orcid":"0000-0001-7034-9513","position":1,"is_corresponding":false},{"id":860447,"name":"Yuexiao Shen","orcid":"0000-0002-8665-2848","position":2,"is_corresponding":false},{"id":668870,"name":"Ji Min Kim","orcid":null,"position":3,"is_corresponding":false},{"id":861134,"name":"Tom A. Tacken","orcid":null,"position":4,"is_corresponding":false},{"id":679760,"name":"Rhesa N. Ledbetter","orcid":"0000-0002-4268-8785","position":5,"is_corresponding":false},{"id":227287,"name":"Sunmoon Yu","orcid":"0000-0001-7250-9365","position":6,"is_corresponding":false},{"id":452631,"name":"Lance C. Seefeldt","orcid":"0000-0002-6457-9504","position":7,"is_corresponding":false},{"id":85277,"name":"Peidong Yang","orcid":"0000-0003-4799-1684","position":8,"is_corresponding":false},{"id":320668,"name":"Stefano Cestellos-Blanco","orcid":"0000-0002-8340-5315","position":0,"is_corresponding":true}],"reference_count":64,"raw_metadata":null,"created_at":"2026-07-19T00:22:21.693802Z","pmid":"35727971","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}