{"doi":"10.1073/pnas.2111173118","title":"Absolute quantification of tumor antigens using embedded MHC-I isotopologue calibrants","abstract":"Absolute quantification measurements (copies per cell) of peptide major histocompatibility complex (pMHC) antigens are necessary to inform targeted immunotherapy drug design; however, existing methods for absolute quantification have critical limitations. Here, we present a platform termed SureQuant-IsoMHC, utilizing a series of pMHC isotopologues and internal standard-triggered targeted mass spectrometry to generate an embedded multipoint calibration curve to determine endogenous pMHC concentrations for a panel of 18 tumor antigens. We apply SureQuant-IsoMHC to measure changes in expression of our target panel in a melanoma cell line treated with a MEK inhibitor and translate this approach to estimate antigen concentrations in melanoma tumor biopsies.","journal":"Proceedings of the National Academy of Sciences","year":2021,"id":162008,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":52,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9533,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":647799,"name":"Aaron S. Gajadhar","orcid":"0000-0002-1200-6608","position":1,"is_corresponding":false},{"id":678144,"name":"Bhavin Patel","orcid":"0000-0002-7050-906X","position":2,"is_corresponding":false},{"id":647800,"name":"Sébastien Gallien","orcid":"0000-0002-3544-992X","position":3,"is_corresponding":false},{"id":399,"name":"Dennie T. Frederick","orcid":"0000-0003-3355-5267","position":4,"is_corresponding":false},{"id":411,"name":"Genevieve M. Boland","orcid":"0000-0002-7522-6173","position":5,"is_corresponding":false},{"id":412,"name":"Ryan J. Sullivan","orcid":"0000-0001-5344-6645","position":6,"is_corresponding":false},{"id":225709,"name":"Forest M. White","orcid":"0000-0002-1545-1651","position":7,"is_corresponding":false},{"id":279214,"name":"Lauren Stopfer","orcid":"0000-0001-6984-3004","position":0,"is_corresponding":true}],"reference_count":12,"raw_metadata":null,"created_at":"2026-07-18T23:45:00.600065Z","pmid":"34497125","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}