{"doi":"10.1073/pnas.2105370118","title":"Let-7b-5p in vesicles secreted by human airway cells reduces biofilm formation and increases antibiotic sensitivity of <i>P. aeruginosa</i>","abstract":"Significance Pseudomonas aeruginosa is one of the leading causes of hospital-acquired infections worldwide primarily due to its ability to develop antibiotic-resistant biofilms. This report describes a mechanism whereby human airway epithelial cells (AECs) increase the ability of antibiotics to kill P. aeruginosa and reduce the formation of drug-resistant biofilms. We demonstrate that human AECs secrete extracellular vesicles containing the microRNA let-7b-5p, that the vesicles deliver let-7b-5p to P. aeruginosa , and that let-7b-5p decreases biofilm formation and increases the ability of beta-lactam antibiotics to reduce biofilm formation by down-regulating key antibiotic-resistance and biofilm genes. A combination of let-7b, which is in clinical trials as an anti-inflammatory agent, and beta-lactam antibiotics may help combat antibiotic-resistant P. aeruginosa infections.","journal":"Proceedings of the National Academy of Sciences","year":2021,"id":154891,"datarank":0.62147020895873,"base_score":4.143134726391533,"endowment":4.143134726391533,"self_citation_contribution":0.62147020895873,"citation_network_contribution":0.0,"self_endowment_contribution":0.62147020895873,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":62,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9546,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":474432,"name":"Amanda Nymon","orcid":null,"position":1,"is_corresponding":false},{"id":65140,"name":"BRUCE A. STANTON","orcid":"0000-0002-1661-407X","position":2,"is_corresponding":false},{"id":655832,"name":"Laura Bashor","orcid":"0000-0002-8609-1219","position":3,"is_corresponding":false},{"id":655833,"name":"Zhongyou Li","orcid":"0000-0002-0196-2979","position":4,"is_corresponding":false},{"id":42556,"name":"Thomas H. Hampton","orcid":"0000-0003-0543-402X","position":5,"is_corresponding":false},{"id":656366,"name":"Amanda Liefeld","orcid":null,"position":6,"is_corresponding":false},{"id":397493,"name":"Fred Kolling","orcid":"0000-0002-6178-9901","position":7,"is_corresponding":false},{"id":655834,"name":"Ian S. LaCroix","orcid":"0000-0002-4859-7024","position":8,"is_corresponding":false},{"id":393304,"name":"Scott A. Gerber","orcid":"0000-0002-2964-5051","position":9,"is_corresponding":false},{"id":456,"name":"Deborah A. Hogan","orcid":"0000-0002-6366-2971","position":10,"is_corresponding":false},{"id":655835,"name":"Swetha Kasetty","orcid":"0000-0001-7199-3239","position":11,"is_corresponding":false},{"id":258382,"name":"Carey D. Nadell","orcid":"0000-0003-1751-4895","position":12,"is_corresponding":false},{"id":472045,"name":"Katja Koeppen","orcid":"0000-0003-1094-0621","position":0,"is_corresponding":true}],"reference_count":83,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:43:54.469024Z","pmid":"34260396","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}